Human Granzyme B Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Granzyme B Affinity Purified Polyclonal Ab (100 UG)

货号： AF2906 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2906
别名	CSPB; CTLA-1; CTSGL1; Fragmentin-2; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT
反应种属	Human
应用	Western Blot(0.25 µg/mL) Simple Western(10 µg/mL)
目标/特异性	Detects human Granzyme B in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human Granzyme H and recombinant mouse Granzyme B is observed.
使用方法	Western Blot: 0.25 µg/mL Simple Western: 10 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3002 (Human); 14939 (Mouse)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Granzyme B Gly19-Tyr247 Accession # P10144
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Human Granzyme B is synthesized as a precursor (247 residues) with a signal peptide (residues 1‑18), a pro peptide (residues 19‑20), and a mature chain (residues 21‑247) (4‑6).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Kam, C-M. et al. (2000) Biochim. Biophys. Acta 1477:307. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al., eds., pp. 1549 - 1552. Schmid, J. and C. Weissman (1987) J. Immunol. 139:250. Caputo, A. et al. (1988) J. Biol. Chem. 263:6363. Trapani, J.A. et al. (1988) Proc. Natl. Acad. Sci. USA 85:6924.

参考图片

Detection of Human Granzyme B by Western Blot. Western blot shows lysates of NK‑92 human natural killer lymphoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2906) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Granzyme B at approximately 32-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Granzyme B by Simple Western^TM. Simple Western lane view shows lysates of NK‑92 human natural killer lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Granzyme B at approximately 42 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Granzyme B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2906) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.