Human Biglycan Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human Biglycan Affinity Purified Polyclonal Ab (100 UG)

货号： AF2667 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2667
别名	BGN; biglycan; Bone/cartilage proteoglycan I; bone/cartilage proteoglycan-I; dermatan sulphate proteoglycan I; DSPG1; PGI; PG-S1; SLRR1A; SLRR1Abiglycan proteoglycan; small leucine-rich protein 1A
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human Biglycan in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	633 (Human); 12111 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. LDL particle core enrichment in cholesteryl oleate increases proteoglycan binding and promotes atherosclerosis. Authors: Melchior, John T, Sawyer, Janet K, Kelley, Kathryn, Shah, Ramesh, Wilson, Martha D, Hantgan, Roy R, Rudel, Lawrence J Lipid Res, 2013;54(9):2495-503. Species: Human Sample Type: protein Application: ChIP Resistance of corneal RFUVA-cross-linked collagens and small leucine-rich proteoglycans to degradation by matrix metalloproteinases. Authors: Zhang Y, Mao X, Schwend T, Littlechild S, Conrad G Invest Ophthalmol Vis Sci, 2013;54(2):1014-25. Species: Human Sample Type: protein Application: WB Renal accumulation of biglycan and lipid retention accelerates diabetic nephropathy. Authors: Thompson J, Wilson P, Brandewie K, Taneja D, Schaefer L, Mitchell B, Tannock LR Am. J. Pathol., 2011;179(3):1179-87. Species: Mouse Sample Type: Whole Tissue Application: IHC Frozen Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway. Authors: Gomez D, Al Haj Zen A, Borges LF, Philippe M, Gutierrez PS, Jondeau G, Michel JB, Vranckx R J. Pathol., 2009;218(1):131-42. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Dermatan sulfate proteoglycan biglycan as a potential selectin L/CD44 ligand involved in selective recruitment of peripheral blood CD16(-) natural killer cells into human endometrium. Authors: Kitaya K, Yasuo T J. Leukoc. Biol., 2009;85(3):391-400. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Biglycan Asp38-Lys368 Accession # P21810
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Biglycan is a small secreted proteoglycan belonging to the leucine-rich repeat (LRR) protein family. It contains a central 12 LRR repeat domain flanked by small cysteine clusters at either side. Two glycosaminoglycans (GAG) chains are attached near the amino terminus. Biglycan binds BMP-4 and Chordin to regulate BMP-4 signaling. It may also be involved in collagen fiber assembly. Human Biglycan shares 98% amino acid sequence homology with mouse Biglycan, 97% sequence homology with rat and bovine Biglycan and 96% sequence homology with canine and equine Biglycan.
运输条件	Blue Ice
存放说明	-20℃
参考文献	ICC / IHC Images: Biglycan Osteoblast and Osteoclast Markers Osteocyte Markers Osteogenesis Markers Proteoglycans Proteoglycans and Regulators Wnt Signaling Modulators

参考图片

Biglycan in Human Kidney Array. Biglycan was detected in immersion fixed paraffin-embedded sections of human kidney array using Human Biglycan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2667) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.