Human Galectin-3BP/MAC-2BP Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human Galectin-3BP/MAC-2BP Affinity Purified Polyclonal Ab (100 UG)

货号： AF2226 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2226
别名	90K; Basement membrane autoantigen p105; BTBD17B; galectin-3-binding protein; L3 antigen; Lectin galactoside-binding soluble 3-binding protein; lectin, galactoside-binding, soluble, 3 binding protein; LGALS3BP; M2BP; Mac-2 BP; Mac-2-binding protein; MAC2BP; MAC-2BP; MAC-2-BP; serum protein 90K; Tumor-associated antigen 90K
全称	Galectin-3 Binding Protein
反应种属	Human
应用	Western Blot(0.2 µg/mL) Simple Western(10 µg/mL)
目标/特异性	Detects human Galectin‑3BP/MAC‑2BP in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 15% cross‑reactivity with recombinant mouse MAC‑2BP is observed.
使用方法	Western Blot: 0.2 µg/mL Simple Western: 10 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3959 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. A galectin-3-dependent pathway upregulates interleukin-6 in the microenvironment of human neuroblastoma. Authors: Silverman A, Nakata R, Shimada H, Sposto R, DeClerck Y Cancer Res, 2012;72(9):2228-38. Species: Human Sample Type: Cell Lysates Application: WB Human galectin 3 binding protein interacts with recombinant adeno-associated virus type 6. J. Virol., 2012;86(12):6620-31. Species: Human Sample Type: Serum Application: WB Diverse functions of reactive cysteines facilitate unique biosynthetic processes of aggregate-prone interleukin-31. Authors: Shen M, Siu S, Byrd S, Edelmann KH, Patel N, Ketchem RR, Mehlin C, Arnett HA, Hasegawa H Exp. Cell Res., 2011;317(7):976-93. Species: Human Sample Type: Cell Lysates Application: WB Identification of galectin-3-binding protein as a factor secreted by tumor cells that stimulates interleukin-6 expression in the bone marrow stroma. Authors: Fukaya Y, Shimada H, Wang LC, Zandi E, DeClerck YA J. Biol. Chem., 2008;283(27):18573-81. Species: Human Sample Type: Cell Culture Supernates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Galectin‑3BP/MAC‑2BP Val19-Asp585 Accession # Q08380
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Koths, K. et al. (1993) J. Biol. Chem. 268:14245. Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401. Sasaki, T. et al. (1998) EMBO J. 17:1606. Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228. Muller, S. A. et al. (1999) J. Mol. Biol. 291:801. Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690. Grassadonia, A. et al. (2004) Glycoconj. J. 19:551. Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.

参考图片

Detection of Human Galectin‑3BP/MAC‑2BP by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Galectin‑3BP/MAC‑2BP by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑3BP/MAC‑2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin‑3BP/MAC‑2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.