| 货号 | AF2217 |
| 别名 | CAD4; cadherin 4, type 1, preproprotein; cadherin 4, type 1, R-cadherin (retinal); cadherin-4; CDH4; FLJ22202; FLJ40547; MGC126700; MGC138355; RCAD; R-CAD; R-Cadherin; Retinal cadherin |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Cadherin‑4/R‑Cadherin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) N-Cadherin is observed and less than 1% cross-reactivity with rhCadherin-8, rhCadherin-11, rhCadherin-12, rhCadherin-17, rhE-Cadherin, rhP-Cadherin, and rhVE-Cadherin is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1002 (Human); 12561 (Mouse); 311710 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Cadherin‑4/R‑Cadherin His21-Ala734 (Lys347Trp) Accession # P55283 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The cadherin superfamily is a large family of membrane-associated glycoproteins that engage in both homo- and heterotypic, calcium-dependent, cell-cell adhesion events. The superfamily can be divided into at least four subfamilies based on its member’s extracellular (EC) regions and cytoplasmic domains (1, 2). These include classical cadherins, desmosomal cadherins, protocadherins, and cadherin-like molecules that contain a variable number of EC and transmembrane (TM) domains (1). Cadherin-4, also known as R-Cadherin, is a classical cadherin of 120‑140 kDa (3, 4). Human Cadherin-4 is synthesized as a 916 amino acid (aa) type I transmembrane glycoprotein that contains a 20 aa signal peptide, a 149 aa prosequence, a 565 aa extracellular region (EC), a 22 aa transmembrane segment, and a 160 aa cytoplasmic domain (5, 6). There are five EC cadherin domains that are approximately 110 aa in length. This pattern is consistent with classical cadherin family molecules that are modular in their extracellular region and mediate calcium-dependent cell-cell adhesion through their Ca++-binding repeats (2). One potential Cadherin-4 splice variant involves the preprosegment and shows 32 aa substitution for the N-terminal 124 amino of the full-length precursor (7). The extracellular region of human Cadherin-4 is 96% aa identical to mouse Cadherin-4 extracellular region (3). Cadherin-4 is expressed in vascular smooth muscle (8), pancreatic beta -cells (9), thyroid follicular cells (10), bone marrow Lin- HSCs (11), sensory neurons of the dorsal root ganglia (12), and, possibly, astrocytes and endothelium of the retina (13). As a classic cadherin, Cadherin-4 will form both homodimers and heterodimers with N-cadherin (4, 14). These complexes translate into adhesion multimers in cis‑ and trans-configurations. Such structures serve to both unite adjacent cells, and provide guidance for migrating cells/processes (13). Additionally, R-Cadherin is associated with cell quiescence, as a loss of cell Cadherin-4 expression is correlated with cell proliferation (8). Finally, R-Cadherin is reported to bind to KLRG1 (killer cell lectin-like receptor G1). This inactivates NK cell cytotoxicity, and provides protection for R-Cadherin expressing cells (15). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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