| 货号 | AF2214 |
| 别名 | activator protein 1; AP-1; DKFZp686C0818; FBJ murine osteosarcoma viral oncogene homolog B; G0S3; G0S3G0/G1 switch regulatory protein 3; GOS3; GOSB; MGC42291; oncogene FOS-B; protein fosB | 全称 | FBJ Murine Osteosarcoma Viral Oncogene Homolog B |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human FosB. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2354 (Human); 14282 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human FosB Met1-Leu338 Accession # P53539 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | FosB is a member of the Fos (Finkel Osteogenic Sarcoma) gene family. It is the product of an immediate early gene that heterodimerizes with Jun family members in the AP-1 transcription complex. FosB has two splice forms that are 52-kDa and 36-kDa in size. Each form contains an N-terminal transcription-activating domain and a leucine-zipper region that allows for heterodimerization. The short form lacks a C-terminal transcription-activating domain that is necessary for gene activation. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
FosB/G0S3 in Human Pancreas. FosB/G0S3 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human FosB/G0S3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2214) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
