Human Siglec-10 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human Siglec-10 Affinity Purified Polyclonal Ab (100 UG)

货号： AF2130 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2130
别名	MGC126774; PRO940; sialic acid binding Ig-like lectin 10; sialic acid-binding Ig-like lectin 10; SIGLEC-10; siglec-like gene 2; Siglec-like protein 2; SLG2; SLG2sialic acid binding Ig-like lectin 10 Ig-like lectin 7
全称	Sialic Acid Binding Ig-like Lectin 10
反应种属	Human
应用	Western Blot(0.1 µg/mL) Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human Siglec‑10 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross‑reactivity with recombinant human (rh) Siglec-5 is observed and less than 1% cross-reactivity with rhSiglec-2, rhSiglec-3, rhSiglec-7, rhSiglec-9, and recombinant mouse Siglec-F is observed.
使用方法	Western Blot: 0.1 µg/mL Flow Cytometry: 2.5 µg/10⁶cells Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 1.5 μg/mL of biotinylated 6’‑Sialyllactose-Polyacrylamide to immobilized Recombinant Human Siglec-10 Fc Chimera (Catalog # 2130-SL) coated at 5 µg/mL (100 µL/well). At 30 μg/mL, this antibody will block >90% of the binding.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	89790 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Alteration and acquisition of Siglecs during in vitro maturation of CD34+ progenitors into human mast cells. Authors: Yokoi H, Myers A, Matsumoto K, Crocker PR, Saito H, Bochner BS Allergy, 2006;61(6):769-76. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human Siglec-10 Met17-Thr546 Accession # Q96LC7
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N‑terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5‑11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system (1‑3). Siglec-G is the apparent ortholog of human Siglec-10 (4). The human Siglec-10 cDNA encodes a 697 amino acid (aa) precursor that includes a 16 aa signal sequence, a 534 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 126 aa cytoplasmic domain. The ECD contains one Ig-like V‑type domain and four Ig-like C2-type domains, while the cytoplasmic domain contains two immunoreceptor tyrosine-based inhibitory motifs (ITIM) (5‑8). Five splice variants of human Siglec-10 differ in their deletions within the ECD. A potentially secreted sixth variant contains the Ig-like V-type domain followed by a 45 aa substitution (5‑7, 9). Within the ECD, human Siglec-10 is most closely related to Siglec-5 (42% aa sequence identity). It shares 63% aa sequence identity with mouse Siglec-G. Siglec-10 is expressed on eosinophils, neutrophils, monocytes, and B cells (5, 8) with some splice variants predominating in particular cell types and tissue locations (6, 7, 9). It is up‑regulated on eosinophils in mouse models of allergic respiratory inflammation (10). Siglec-10 binds sialated proteins and lipids in alpha 2,3 or alpha 2,6 linkage and shows a preference for GT1b gangliosides (7, 11). This binding can be modulated by cis interactions of Siglec-10 with sialated molecules expressed on the same cell (7). When tyrosine phosphorylated, the cytoplasmic ITIMs interact with phosphatases SHP-1 and SHP-2 to propagate inhibitory signals (5, 9).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Crocker, P.R. (2005) Curr. Opin. Pharmacol. 5:431. Crocker, P.R. (2002) Curr. Opin. Struct. Biol. 12:609. Crocker, P.R. and J. Zhang (2002) Biochem. Soc. Symp. 69:83. Angata, T. et al. (2001) J. Biol. Chem. 276:45128. Whitney, G. et al. (2001) Eur. J. Biochem. 268:6083. Yousef, G.M. et al. (2001) Biochem. Biophys. Res. Commun. 284:900. Li, N. et al. (2001) J. Biol. Chem. 276:28106. Munday, J. et al. (2001) Biochem. J. 355:489. Kitzig, F. et al. (2002) Biochem. Biophys. Res. Commun. 296:355. Aizawa, H. et al. (2003) Genomics 82:521. Rapoport, E. et al. (2003) Bioorg. Med. Chem. Lett. 13:675.

参考图片

Detection of Siglec‑10 in Human B Cells by Flow Cytometry.

Human whole blood CD19⁺ B cells were stained with Goat Anti-Human Siglec‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2130, filled histogram) or isotype control antibody (Catalog # AB‑108‑C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).