Human GATA-2 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

产品中心

当前位置：首页>产品中心

Human GATA-2 Affinity Purified Polyclonal Ab (100 UG)

货号： AF2046 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2046
别名	endothelial transcription factor GATA-2; FLJ45948; GATA binding protein 2; GATA-binding protein 2NFE1B; MGC2306
反应种属	Human
应用	Western Blot(0.5 µg/mL) Simple Western(5 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human GATA-2. In direct ELISAs and Western blots, this antibody shows less than 1% cross-reactivity with rhGATA-1, rhGATA-5 and rhGATA-6.
使用方法	Western Blot: 0.5 µg/mL Simple Western: 5 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2624 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Multiple mouse models of primary lymphedema exhibit distinct defects in lymphovenous valve development. Authors: Geng X, Cha B, Mahamud M, Lim K, Silasi-Mansat R, Uddin M, Miura N, Xia L, Simon A, Engel J, Chen H, Lupu F, Srinivasan R Dev Biol, 2016;409(1):218-33. Species: Mouse Sample Type: Whole Tissue Application: IHC-OCT embedded GATA2 germline mutations impair GATA2 transcription, causing haploinsufficiency: functional analysis of the p.Arg396Gln mutation. Authors: Cortes-Lavaud X, Landecho M, Maicas M, Urquiza L, Merino J, Moreno-Miralles I, Odero M J Immunol, 2015;194(5):2190-8. Species: Human Sample Type: Whole Cells Application: ChIP The Down syndrome critical region gene 1 short variant promoters direct vascular bed-specific gene expression during inflammation in mice. Authors: Minami T, Yano K, Miura M, Kobayashi M, Suehiro J, Reid PC, Hamakubo T, Ryeom S, Aird WC, Kodama T J. Clin. Invest., 2009;119(8):2257-70. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human GATA-2 Ala15-Thr279 Accession # P23769
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	GATA factors constitute a family of transcriptional regulatory factors that bind to the consensus DNA sequence (A/T) GATA (A/G) to control diverse tissue-specific programs of gene expression and morphogenesis. GATA-1/2/3 are each expressed in the hematopoietic system while GATA 4/5/6 are each expressed in the developing heart and in gastrointestinal and gut-derived tissues (1, 2).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Tsai, S.F. et al. (1989) Nature 339:446. Jiang, Y. and T. Evans (1996) Dev. Biol. 174:258.

参考图片

Detection of Human GATA‑2 by Western Blot. Western blot shows lysates of NIH‑3T3 mouse embryonic fibroblast cell line and KG‑1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 0.5 µg/mL of Human GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GATA‑2 at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human GATA‑2 by Simple Western^TM. Simple Western lane view shows lysates of KG‑1 human acute myelogenous leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for GATA‑2 at approximately 64 kDa (as indicated) using 5 µg/mL of Goat Anti-Human GATA‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2046) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.