Human Complement C5/C5a Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human Complement C5/C5a Affinity Purified Polyclonal Ab (100 UG)

货号： AF2037 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2037
别名	C5
反应种属	Human
应用	Western Blot(0.1 µg/mL)
目标/特异性	Detects human Complement Component C5/C5a in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant mouse Complement Component C5a is observed.
使用方法	Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize Complement Component C5a-induced chemotaxis in the J774A.1 mouse reticulum cell sarcoma macrophage cell line [Zwirner, J. et al. (1998) Eur. J. Immunol. 28:1570]. The Neutralization Dose (ND₅₀) is typically 0.075-0.4 µg/mL in the presence of 15 ng/mL Recombinant Human Complement Component C5a.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	727 (Human); 15139 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Production of C5a by ASP, a serine protease released from Aeromonas sobria. Authors: Nitta H, Imamura T, Wada Y, Irie A, Kobayashi H, Okamoto K, Baba H J. Immunol., 2008;181(5):3602-8. Species: Human Sample Type: Plasma Application: WB
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human Complement Component C5a Thr678-Arg751 Accession # P01031
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Human complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 alpha -chain (1, 2). Human C5a has four alpha -helices plus three intrachain disulfide bonds that create a triple loop structure (3). In serum, proteolytic processing removes the C-terminal arginine, creating a low activity C5adesArg74 molecule (1). Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2 (4). Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation (1, 5). It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Gerard, C. and N.P. Gerard (1994) Annu. Rev. Immunol. 12:775. DiScipio, R.G. et al. (1983) J. Biol. Chem. 258:10629. Huber-Lang, M.S. et al. (2003) J. Immunol. 170:6115. Okinaga, S. et al. (2003) Biochemistry 42:9406. Gerard, N.P. and C. Gerard (2002) Curr. Opin. Immunol. 14:705.

参考图片

Chemotaxis Induced by Complement Component C5a and Neutralization by Human Complement Component C5a Antibody. Recombinant Human Complement Component C5a (Catalog # 2037-C5) chemoattracts the J774A.1 mouse reticulum cell sarcoma macrophage cell line in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human Complement Component C5a (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human Complement Component C5a Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2037). The ND₅₀ is typically 0.075-0.4 µg/mL.