Human RUNX2/CBFA1 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human RUNX2/CBFA1 Affinity Purified Polyclonal Ab (100 UG)

货号： AF2006 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2006
别名	Acute myeloid leukemia 3 protein; AML3CCD; CBFA1; CBFA1MGC120022; Core-binding factor subunit alpha-1; MGC120023; PEA2-alpha A; PEBP2A; PEBP2A1CCD1; PEBP2aA1; PEBP2-alpha A; runt domain, alpha subunit 1; runt-related transcription factor 2; SL3/AKV core-binding factor alpha A subunit; SL3-3 enhancer factor 1 alpha A subunit
全称	Runt-related Transcription Factor 2
反应种属	Human
应用	Western Blot(0.1 µg/mL) Chromatin Immunoprecipitation (ChIP)(5 µg/5 x 10⁶cells) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects human RUNX2/CBFA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 3% cross-reactivity with recombinant human (rh) RUNX1 and rhRUNX3 is observed.
使用方法	Western Blot: 0.1 µg/mL Chromatin Immunoprecipitation (ChIP): 5 µg/5 x 10⁶cells Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	860 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Histone Deacetylase Inhibitors Repress Tumoral Expression of the Proinvasive Factor RUNX2. Authors: Sancisi V, Gandolfi G, Ambrosetti D, Ciarrocchi A Cancer Res, 2015;75(9):1868-82. Species: Human Sample Type: Cell Lysates Application: WB Differential requirements for IKKalpha and IKKbeta in the differentiation of primary human osteoarthritic chondrocytes. Authors: Olivotto E, Borzi RM, Vitellozzi R, Pagani S, Facchini A, Battistelli M, Penzo M, Li X, Flamigni F, Li J, Falcieri E, Facchini A, Marcu KB Arthritis Rheum., 2008;58(1):227-39. Species: Human Sample Type: Whole Cells Application: ICC
纯化方式	Antigen Affinity-purified
免疫原	E. coli-derived recombinant human RUNX2/CBFA1 isoform 2 Lys219-Tyr404 Accession # Q13950
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Core Binding Factor A1 (CBFA1; also called AML-3, PEBP-2 alpha A, OSF-2, RUNX2) is a 56 kDa DNA-binding protein that belongs to the Runt-related family of transcription factors. It is synthesized as a 521 amino acid (aa) protein with multiple domains. From the N-terminus, there is a 19 aa activation domain (AD1), a 41 aa glutamine/alanine (Q/A) repeat segment (AD2), a 128 aa Runt/DNA-binding region, a 9 aa NLS and a 285 aa ProSerThr (PST)-rich sequence that is needed for gene activation. Two isoforms exist, one with a truncated AD1 domain and another with a 22 aa deletion in the AD3 section of PST-rich sequence. Human CBFA1 is 98% aa identical to mouse CBFA1.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Epithelial Stem Cell Transcription Factors Mesenchymal Stem Cell Transcription Factors Notch Pathway Osteoblast and Osteoclast Markers Osteogenesis Markers Osteoprogenitor Markers Transcription Factors and Regulators Transcription Factors in the Akt Pathway

参考图片

Detection of RUNX2/CBFA1-regulated Genes by Chromatin Immunoprecipitation. Jurkat human acute T cell leukemia cell line treated with 50 ng/mL PMA and 200 ng/mL calcium ionomycin overnight was fixed using formaldehyde, resuspended in lysis buffer, and sonicated to shear chromatin. RUNX2/CBFA1/DNA complexes were immunoprecipitated using 5 μg Goat Anti-Human RUNX2/CBFA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2006) or control antibody (Catalog # AB-108-C) for 15 minutes in an ultrasonic bath, followed by Biotinylated Anti-Goat IgG Secondary Antibody (Catalog # BAF109). Immunocomplexes were captured using 50 μL of MagCellect Streptavidin Ferrofluid (Catalog # MAG999) and DNA was purified using chelating resin solution. The VEGF promoter was detected by standard PCR.