Human CD46 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human CD46 Affinity Purified Polyclonal Ab (100 UG)

货号： AF2005 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF2005
别名	AHUS2; CD46 antigen; CD46 molecule, complement regulatory protein; CD46; MCP; membrane cofactor protein (CD46, trophoblast-lymphocyte cross-reactive antigen); MIC10; TLXcomplement regulatory protein; trophoblast leucocyte common antigen; Trophoblast leukocyte common antigen; trophoblast-lymphocyte cross-reactive antigen
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells) Immunohistochemistry(1-15 µg/mL)
目标/特异性	Detects human CD46 in direct ELISAs and Western blots.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells Immunohistochemistry: 1-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	4179 (Human); 17221 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Preclinical safety, pharmacokinetics, pharmacodynamics, and biodistribution studies with Ad35K++ protein: a novel rituximab cotherapeutic Authors: M Richter, R Yumul, K Saydaminov, H Wang, M Gough, A Baldessari, R Cattaneo, F Lee, CH Wang, H Jang, A Astier, A Gopal, D Carter, A Lieber Mol Ther Methods Clin Dev, 2016;5(0):16013. Species: Mouse Sample Type: Serum Influence of coagulation factor x on in vitro and in vivo gene delivery by adenovirus (Ad) 5, Ad35, and chimeric Ad5/Ad35 vectors. Authors: Greig JA, Buckley SM, Waddington SN, Parker AL, Bhella D, Pink R, Rahim AA, Morita T, Nicklin SA, McVey JH, Baker AH Mol. Ther., 2009;17(10):1683-91. Species: Human Sample Type: Tissue Homogenates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human CD46 Cys35-Asn328 Accession # NP_722548
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	CD46, also known as MCP (complement membrane cofactor protein), is a type I membrane protein that has cofactor activity for inactivation of complement components C3b and C4b by serum factor I. CD46 is widely distributed in white blood cells, platelets, epithelial cells and fibroblasts. At least fourteen different transcript variants encoding different isoforms have been reported for this gene.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Complement Pathway Epithelial Cell Markers and Intracellular Molecules Human CD Marker Products ICC / IHC Images: CD46 Surface Ectodermal Cells

参考图片

Detection of Human CD46 by Western Blot. Western blot shows lysate of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD46 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2005) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CD46 at approximately 50-70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

CD46 in Human Skin. CD46 was detected in immersion fixed paraffin-embedded sections of human skin using 1.7 µg/mL Goat Anti-Human CD46 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2005) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.