| 货号 | AF1987 |
| 别名 | 16.3A5; 1F5 antigen; 1F5; 20 kDa homologous restriction factor; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16; CD59 antigen; CD59 antigen, complement regulatory protein; CD59 glycoprotein; CD59 molecule, complement regulatory protein; CD59; EJ16; EJ30; EJ30, EL32 and G344); EL32; FLJ38134; FLJ92039; G344; HRF20; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MACIF; MAC-inhibitory protein; MAC-IP; MEM43 antigen; MEM43; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MGC2354; MIC11; MIC11MSK21; MIN1; MIN2; MIN3; MIRL; p18-20; Protectin; surface anitgen recognized by monoclonal 16.3A5; T cell-activating protein |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(50 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human CD59 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 50 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 966 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Apolipoprotein J/Clusterin in Human Erythrocytes Is Involved in the Molecular Process of Defected Material Disposal during Vesiculation. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human CD59 Leu26-Asn102 Accession # P13987 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | CD59 is a GPI-anchored membrane glycoprotein that functions to inhibit formation of the complement membrane attack complex and thereby prevent cell injury. CD59 is expressed on erythrocytes and on all peripheral blood leukocytes. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human CD59 by Western Blot. Western blot shows lysates of human breast tissue and HUVEC human umbilical vein endothelial cells. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for CD59 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
CD59 in Human Breast. CD59 was detected in immersion fixed paraffin-embedded sections of human breast using 1.7 µg/mL Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human CD59 by Simple WesternTM. Simple Western lane view shows lysates of HUVEC human umbilical vein endothelial cells, loaded at 0.2 mg/mL. A specific band was detected for CD59 at approximately 26 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CD59 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1987) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
