Human IL-24 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human IL-24 Affinity Purified Polyclonal Ab (100 UG)

货号： AF1965 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF1965
别名	C49A; FISP; IL10B; IL-24; interleukin 24; MDA-7; MDA7MOB5; melanocyte-associated Mda-7; melanoma differentiation association protein 7; Melanoma differentiation-associated gene 7 protein; MOB-5; ST16; ST16interleukin-24; suppression of tumorigenicity 16 (melanoma differentiation); Suppression of tumorigenicity 16 protein
全称	Interleukin 24
反应种属	Human
应用	Western Blot(0.1 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human IL-24 in direct ELISAs and Western blots.
使用方法	Western Blot: 0.1 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells Neutralization: Measured by its ability to neutralize IL‑24-induced proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta. The Neutralization Dose (ND₅₀) is typically 0.2-0.6 µg/mL in the presence of 2 ng/mL Recombinant Human IL‑24.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	11009 (Human); 93672 (Mouse); 170819 (Rat)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Induction of phenotype modifying cytokines by FERMT1 mutations. Authors: Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C Hum. Mutat., 2011;32(4):397-406. Species: Human Sample Type: Whole Cells Application: Neut Interleukin-24 inhibits the plasma cell differentiation program in human germinal center B cells. Authors: Maarof G, Bouchet-Delbos L, Gary-Gouy H, Durand-Gasselin I, Krzysiek R, Dalloul A Blood, 2010;115(9):1718-26. Species: Human Sample Type: Whole Tissue Application: IHC Frozen Systemic cancer gene therapy using adeno-associated virus type 1 vector expressing MDA-7/IL24. Authors: Tahara I, Miyake K, Hanawa H, Kurai T, Hirai Y, Ishizaki M, Uchida E, Tajiri T, Shimada T Mol. Ther., 2007;15(10):1805-11. Species: Human Sample Type: Plasma Application: ELISA Development
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human IL‑24 Gln50-Leu206 Accession # Q2YHE5
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Interleukin 24 (IL-24), also known as mda-7 (melanoma differentiation associated gene-7), is a member of the IL-10 family of helical cytokines. The IL-24 gene encodes a precursor protein of 207 amino acids that contains a 48 amino acid (aa) signal sequence and an 18 kDa, 158 aa mature segment. There are three potential N-linked glycosylation sites, at least one of which is used. When secreted, IL-24 is a 35-40 kDa phosphorylated glycoprotein that apparently can exist as either a monomer or dimer. It is suggested that glycosylation is essential for activity. Mature human IL-24 shares 69% aa sequence identity with mouse and rat IL-24. Human IL-24 is also active in rodent systems. Cells known to express IL-24 include B cells, CD4⁺ T cells, NK cells, lymph node dendritic cells, monocytes, melanocytes, and melanoma cells. Functionally, IL-24 has diverse activities. At low concentrations on monocytes, it induces type I proinflammatory cytokines such as IFN-gamma, IL-1 beta, IL‑12, and TNF-alpha. At high concentrations, it is a strong inducer of apoptosis in tumor cells but not normal cells. IL-24 also has anti-angiogenic properties. It directly binds IL-24 receptors on endothelial cells, activating STAT3 and blocking their differentiation. IL-24 binds and signals through two heterodimeric receptor complexes. One complex is the combination of IL-20 R alpha and IL-20 R beta, which is shared with IL-19 and IL-20. The second complex is a combination of IL-22 R and IL-20 R beta, which is shared with IL-20.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Jiang, H. et al. (1995) Oncogene 11:2477. Jiang, H. et al. (1996) Proc. Natl. Acad. Sci. USA 93:9160. Wang, M. et al. (2002) J. Biol Chem. 227:7341. Chada, S. et al. (2004) Int. Immunopharmacol. 4:649. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. Chen, J. et al. (2003) Molec. Ther. 8:220.

参考图片

Cell Proliferation Induced by IL‑24 and Neutralization by Human IL‑24 Antibody. Recombinant Human IL‑24 (Catalog # 1965-IL) stimulates proliferation in the BaF3 mouse pro‑B cell line co-transfected with human IL‑20 R alpha and IL‑20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑24 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑24 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1965). The ND₅₀ is typically 0.2-0.6 µg/mL.