A novel ELISA for measuring CD36 protein in human adipose tissue.
Authors: Allred CC, Krennmayr T, Koutsari C, Zhou L, Ali AH, Jensen MD
J. Lipid Res., 2011;52(2):408-15.
Species: Human
Sample Type: Cell Lysates
Application: WB
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis.
Authors: Xu G, Watanabe T, Iso Y, Koba S, Sakai T, Nagashima M, Arita S, Hongo S, Ota H, Kobayashi Y, Miyazaki A, Hirano T
Circ. Res., 2009;105(5):500-10.
Species: Human
Sample Type: Cell Lysates
Application: WB
Internal mammary artery smooth muscle cells resist migration and possess high antioxidant capacity.
Authors: Mahadevan VS, Campbell M, McKeown PP, Bayraktutan U
Cardiovasc. Res., 2006;72(1):60-8.
Species: Human
Sample Type: Cell Lysates
Application: WB

CD36, alternatively known as platelet membrane glycoprotein IV (GPIV), GPIIIb, thrombospondin receptor, collagen receptor, fatty acid translocase (FAT), and scavenger receptor class B, member 3 (SR-B3), is an integral membrane glycoprotein that has multiple physiological functions (1). It is broadly expressed on a variety of cell types including microvascular endothelium, adipocytes, skeletal muscle, epithelial cells of the retina, breast, and intestine, smooth muscle cells, erythroid precursors, platelets, megakaryocytes, dendritic cells, monocytes/macrophages, and microglia (1, 2). As a member of the scavenger receptor family, CD36 is a multiligand pattern recognition receptor that interacts with a large number of structurally dissimilar ligands, including long chain fatty acid (LCFA), advanced glycation end products (AGE), thrombospondin-1, oxidized low-density lipoproteins (oxLDLs), high density lipoprotein (HDL), phosphatidylserine, apoptotic cells, beta -amyloid fibrils (fA beta ), collagens I and IV, and Plasmodium falciparum-infected erythrocytes (3). CD36 is required for the anti-angiogenic effects of thrombospondin-1 in the corneal neovascularization assay (4). It plays a role in lipid metabolism and has been identified as a fatty acid translocase necessary for the binding and transport of LCFA in cells and tissues (5). CD36 has been implicated in the clearance of apoptotic cells and cell debris and has also been shown to mediate the internalization and degradation of a variety of its ligands such as oxLDL, AGE and fA beta (3). Upon ligand binding, CD36 transduces signals that mediate a wide range of pro-inflammatory cellular responses (2). CD36 plays a significant role in the initiation and pathogenesis of chronic inflammatory diseases such as Alzheimer’s disease and atherosclerosis (2, 3). The human CD36 gene encodes a single-chain 472 amino acid residue protein containing both an N- and a C-terminal cytoplasmic tail and an extracellular loop.

1. Febbraio, M. et al. (2001) J. Clin. Invest. 108:785. 
2. Khoury, J. et al. (2003) J. Exp. Med. 197:1657. 
3. Husemann, J. et al. (2002) Glia 40:195.
4. Armstrong, L and P. Bornstein (2003) Matrix. Biol. 22:63.
5. Febbraio M. et al. (1999) J. Biol. Chem. 274:19055.