| 货号 | AF1927 |
| 别名 | BMP-1; bone morphogenetic protein 1; EC 3.4.24; EC 3.4.24.19; FLJ44432; Mammalian tolloid protein; mTld; PCOLC; PCP; PCP2; procollagen C-endopeptidase; Procollagen C-proteinase; TLD | 全称 | Bone Morphogenetic Protein 1 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human BMP‑1/PCP direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) TLL-2 is observed, and less than 1% cross-reactivity with rhBMP-2, -3, -3b, -4, -5, -6, -7, -8, -9, -10, and -15 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 649 (Human) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Defective Proteolytic Processing of Fibrillar Procollagens and Prodecorin Due to Biallelic BMP1 Mutations Results in a Severe, Progressive Form of Osteogenesis Imperfecta. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human BMP‑1/PCP Ala121-Gln730 Accession # NP_001190 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bone morphogenetic protein 1 (BMP-1), also known as procollagen C-proteinase (PCP), is a zinc protease of the astacin family (1, 2). BMP-1/PCP plays a key role in formation of extracellular matrix (ECM) by converting precursor proteins into their mature and functional forms. The precursor proteins identified as substrates for BMP-1/PCP include collagens, biglycan, laminin 5, dentin matrix protein-1, and lysyl oxidase (3). There are six alternatively spliced forms known to be derived from the BMP-1 gene, and isoform 1 consisting of residues 1 to 730 was expressed. The secreted and purified protein does not contain the signal peptide (amino acid residues 1‑22) and pro domain (residues 23‑120), but contain protease (residues 121‑321), CUB I (residues 322‑434), CUB II (residues 435‑546), EGF-like (residues 547‑588) and CUB III (residues 591‑703) domains. The pro domain is apparently cleaved by a furin-like proprotein convertase (4). The purified BMP-1/PCP is an active protease and its peptidase activity can be determined as described above. The purified BMP-1/PCP is predicted to possess procollagen C-proteinase activity because it contains the minimal domain structure required (5). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Recombinant Human BMP‑1/PCP by Western Blot. Western blot shows 25 ng of Recombinant Human BMP‑1/ PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human Meprin alpha Subunit/MEP1A (Catalog # 3220-ZN), Recombinant Human Meprin beta Subunit/MEP1B (Catalog # 2895-ZN), Recombinant Human Ovastacin, Recombinant Human ADAM10 (Catalog # 936-AD), Recombinant Human ADAMTS4 (Catalog # 4307-AD), and Recombinant Human MMP‑1 (Catalog # 901-MP). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP‑1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP‑1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. | |
Detection of Recombinant Human BMP‑1/PCP by Western Blot. Western blot shows 25 ng of Recombinant Human BMP‑1/ PCP (Catalog # 1927-ZN), Recombinant Human TLL-2, Recombinant Human/Mouse/ Rat BMP‑2 (Catalog # 355-BM), Recombinant Human BMP‑3 (Catalog # 113-BP), Recombinant Human BMP‑3b/GDF‑10 (Catalog # 1543-BP), Recombinant Human BMP‑4 (Catalog # 314-BP), Recombinant Human BMP‑5 (Catalog # 615-BMC), Recombinant Human BMP‑6 (Catalog # 507-BP), Recombinant Human BMP‑7 (Catalog # 354-BP), Recombinant Human BMP‑8a (Catalog # 1073-BP), Recombinant Human BMP‑8b (Catalog # 9316-BP), Recombinant Human BMP‑9 (Catalog # 3209-BP), Recombinant Human BMP‑10 (Catalog # 2926-BP) and Recombinant Human BMP‑15/GDF‑9B (Catalog # 5096-BM). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human BMP‑1/PCP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1927) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BMP‑1/PCP at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3. |
