Human ECE-1 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human ECE-1 Affinity Purified Polyclonal Ab (100 UG)

货号： AF1784 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF1784
别名	EC 3.4.24; EC 3.4.24.71; ECE; ECE-1; endothelin converting enzyme 1; endothelin-converting enzyme 1
全称	Endothelin-converting Enzyme-1
反应种属	Human
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) Intracellular Staining by Flow Cytometry(2.5 µg/10⁶cells)
目标/特异性	Detects human ECE-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human ECE-2 is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL Intracellular Staining by Flow Cytometry: 2.5 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	1889 (Human)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Endothelin-converting enzyme-1 (ECE-1) is post-transcriptionally regulated by alternative polyadenylation. Authors: Whyteside A, Turner A, Lambert D PLoS ONE, 2014;9(1):e83260. Species: Human Sample Type: Cell Lysates Application: WB Expression and functional profiling of neprilysin, insulin-degrading enzyme, and endothelin-converting enzyme in prospectively studied elderly and Alzheimer's brain. Authors: Wang S, Wang R, Chen L, Bennett DA, Dickson DW, Wang DS J. Neurochem., 2010;115(1):47-57. Species: Human Sample Type: Tissue Homogenates Application: WB Endosomal endothelin-converting enzyme-1: a regulator of beta-arrestin-dependent ERK signaling. Authors: Cottrell GS, Padilla BE, Amadesi S, Poole DP, Murphy JE, Hardt M, Roosterman D, Steinhoff M, Bunnett NW J. Biol. Chem., 2009;284(33):22411-25. Species: Mouse Sample Type: Whole Tissue Application: IHC Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. Authors: Padilla BE, Cottrell GS, Roosterman D, Pikios S, Muller L, Steinhoff M, Bunnett NW J. Cell Biol., 2007;179(5):981-97. Species: Human Sample Type: Cell Lysates Application: WB
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human ECE-1 Gln90-Trp770 Accession # P42892
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Endothelin-converting Enzyme 1 (ECE-1) is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, Kell and several NEP-like proteins (1). ECE-1 is a type II transmembrane protein with a short cytoplasmic tail and a large ectodomain. Four alternatively spliced isoforms differ in their cytoplasmic tail (2, 3). In addition to big endothelin-1, ECE-1 cleaves a variety of bioactive peptides such as bradykinin, neurotensin, angiotensin I, and substance P (1). Together with ECE-2, it is also involved in degradation of beta -amyloid peptide (4). The ectodomain of human ECE-1, which is common to all isoforms, was expressed with an N-terminal His tag and purified.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Turner, A.J. et al. (2001) BioEssays 23:261. Valdennaire, O. et al. (1999) Eur. J. Biochem. 264:341. Schweizer, A. et al. (1997) Biochem. J. 328:871. Eckman, E.A. et al. (2003) J. Biol. Chem. 278:2081.

参考图片

Detection of ECE‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with Human ECE‑1 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF1784, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

ECE‑1 in Human Kidney. ECE‑1 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human ECE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1784) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the endothelial cells in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.