| 货号 | AF1658 |
| 别名 | GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin-like protein 1; podocalyxin-like; PODXL |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Flow Cytometry(2.5 µg/106cells) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Podocalyxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse Podocalyxin and recombinant human Endoglycan. |
| 使用方法 | Western Blot: 1 µg/mL Flow Cytometry: 2.5 µg/106cells Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5420 (Human); 27205 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. ?-Catenin Is Required for Endothelial Cyp1b1 Regulation Influencing Metabolic Barrier Function | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human Podocalyxin Ser23-Arg427 Accession # AAB61574 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Podocalyxin by Western Blot. Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Podocalyxin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Podocalyxin in Human Kidney. Podocalyxin was detected in immersion fixed paraffin-embedded sections of human kidney using 5 µg/mL Goat Anti-Human Podocalyxin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1658) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to podocytes in glomeruli. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
