Human SLAM/CD150 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Human SLAM/CD150 Affinity Purified Polyclonal Ab (100 UG)

货号： AF164 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AF164
别名	CD 150; CD150; CD150IPO-3; CDw150; IPO-3; signaling lymphocytic activation molecule family member 1; signaling lymphocytic activation molecule; SLAMCD150 antigen; SLAMF1
全称	Signaling Lymphocytic Activation Molecule
反应种属	Human
应用	Western Blot(1 µg/mL) Flow Cytometry(0.25 µg/10⁶cells)
目标/特异性	Detects human SLAM/CD150 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse SLAM, recombinant human (rh) CD48, rhCD84, and rh2B4/CD244 is observed.
使用方法	Western Blot: 1 µg/mL Flow Cytometry: 0.25 µg/10⁶cells
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6504 (Human)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant human SLAM/CD150 Thr25-Pro237 Accession # Q13291
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	SLAM (also IPO-3 and CD150) is a 70‑80 kDa member of the CD2 family of molecules. It is a costimulatory receptor that mediates T cell activation via SAP. SLAM is found on B cells, dendritic cells, macrophages, stem cells and Th1 T cells. SLAM engages in homophilic interactions. Mature human SLAM is 315 amino acids (aa) in length. It is a type I transmembrane glycoprotein whose extracellular domain contains one V-type and one C2-type Ig-like domain (aa 21‑237). There is a 77 aa SH2‑binding cytoplasmic domain. Four potential splice variants are suggested. One shows a deletion of aa 234‑263 (potentially soluble), while three others show aa substitutions; i.e.‑a ten aa substitution for aa 289‑335, a six aa substitution for aa 264‑335 and a 20 aa substitution for aa 125‑335 (potentially soluble). Over aa 1‑236, human SLAM shares 60% aa identity with mouse SLAM.
运输条件	Blue Ice
存放说明	-20℃
参考文献	B Cell Activation Co-stimulatory and Co-inhibitory Molecules Co-stimulatory Molecules Associated with Tfh Cells Dendritic Cell Co-stimulatory Molecules Dendritic Cell ITIM/ITAM Immunoreceptors Endothelial Cell Markers Hematopoietic Stem Cell Markers Human CD Marker Products ITIM/ITAM Immunoreceptors and Related Molecules Lymphoma Macrophage Activation Markers Megakaryocytes Monocyte Markers Natural Killer T (NKT) Cells Platelets Regulatory T Cells (Tregs)

参考图片

Detection of SLAM/CD150 in Human Lymphocytes by Flow Cytometry. Human whole blood lymphocytes were stained with Sheep Anti-Human SLAM/CD150 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF164, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # NL010).

Detection of Human SLAM/CD150 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Nalm‑6 human Pre‑B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SLAM/CD150 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF164) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SLAM/CD150 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.