| 货号 | AF1208 |
| 别名 | NgR; NgR1; Nogo receptor; Nogo-66 receptor; NOGORUNQ330/PRO526; reticulon 4 receptor; reticulon-4 receptor; RTN4R | 全称 | Nogo-66 Receptor/Reticulon 4 Receptor |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human Nogo Receptor (NgR) in direct ELISAs and Western blots. In these formats, approximately 25% cross-reactivity with recombinant mouse NgR is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 1-4 µg/mL of this antibody will block 50% of the binding of 1 µg/mL of Recombinant Rat MAG/Siglec-4a Fc Chimera (Catalog # 538-MG) to immobilized Recombinant Rat Nogo R Fc Chimera (Catalog # 1208-NG) coated at 5 µg/mL (100 µL/well). At 100 µg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 65078 (Human); 65079 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Nogo-66 inhibits adhesion and migration of microglia via GTPase Rho pathway in vitro. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | recombinant human NgR Cys27-Ser447 Accession # Q9BZR6 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Nogo Receptor (NgR), also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the family of leucine-rich repeat (LRR) proteins (1). It is expressed predominantly in the central nervous systems in neurons and their axons. NgR plays an essential role in mediating axon growth inhibition induced by the structurally distinct myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp) (2, 3). Human NgR cDNA encodes a 473 amino acid residues (aa) precursor with a 26 aa putative signal peptide, an LRR-type N-terminal region, eight LRR repeats, a cysteine-rich LRR-type C-terminal region, a GPI linkage domain and a 26 aa C-terminal propeptide that is removed in the mature form (1). All of the LRR domains within NgR are required for ligand binding and receptor oligomerization (4). NgR mediates its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a tumor necrosis factor receptor superfamily (TNFRSF) member also known for modulating the activities of the Trk family of receptor tyrosine kinases, and for inducing apoptosis in neurons and oligodendrocytes (5). Upon ligand binding, NgR binds to and activates the p75NTR. The activated p75NTRthen sequesters the Rho guanine dissociation inhibitor (Rho-GDI) away from Rho and allows Rho to change into the active GTP-bound state which can interact with signaling proteins to suppress axonal growth and regeneration (4). The truncated extracellular domain of NgR has been shown to bind the myelin-derived inhibitors and block inhibition of axon growth by myelin (6). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|