| 货号 | AF1177 |
| 别名 | Cell proliferation-inducing gene 35 protein; EPC-1; EPC-1PIG35; PEDF; PEDFpigment epithelium-derived factor; pigment epithelium derived factor), member 1; proliferation-inducing protein 35; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin; Serpin F1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epitheliumderived factor), member 1 | 全称 | Serpine F1/Pigment Epithelium-derived Factor |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Simple Western(2 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human Serpin F1/PEDF in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse Serpin F1 is observed. |
| 使用方法 | Western Blot: 0.2 µg/mL Simple Western: 2 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 5176 (Human); 20317 (Mouse); 287526 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Pigment epithelium-derived factor (PEDF) binds to caveolin-1 and inhibits the pro-inflammatory effects of caveolin-1 in endothelial cells. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Serpin F1/PEDF Asp44-Pro418 Accession # P36955 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Serpin F1 (serineproteinaseinhibitor-cladeF #1; also PEDF, PIG35 and EPC-1) is a secreted, 50 kDa glycoprotein member of the clade F-subfamily, serpin superfamily of protease inhibitors. It is expressed by diverse cell types such as retinal pigment and breast epithelium, fibroblasts, astrocytes and hepatocytes. Serpin F1 circulates in blood and binds to type I collagen plus heparan sulfate. Although it is a serpin, it belongs to a class of serpins that are nonprotease inhibiting, and instead is known to possess potent antiangiogenic and neurotrophic activity. Human Serpin F1 is 418 amino acids (aa) in length. It contains a 19 aa signal sequence plus a 399 aa mature region that shows an NLS (aa 146-149), a neuroprotective motif (aa 354-359) and an antiangiogenesis segment (aa 387‑411). Phosphorylation occurs at multiple sites that affect bioactivity. Over aa 44‑418, human Serpin F1 shares 87% aa identity with mouse Serpin F1. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Serpin F1/PEDF by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, human liver tissue, and human breast tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Serpin F1/PEDF at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Serpin F1/PEDF by Simple WesternTM. Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Serpin F1/PEDF at approximately 56 kDa (as indicated) using 2 µg/mL of Goat Anti-Human Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. | |
Serpin F1/PEDF in Human Kidney. Serpin F1/PEDF was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human Serpin F1/PEDF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1177) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
