Mouse CCL2/JE/MCP-1 Polyclonal Ab (1 MG)【科瑞奥博】

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Mouse CCL2/JE/MCP-1 Polyclonal Ab (1 MG)

货号： AB-479-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-479-NA
别名	MCAF; MCP-1
反应种属	Mouse
应用	Western Blot(1 µg/mL)
目标/特异性	Detects mouse CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human MCP-1, recombinant mouse MCP-5, and recombinant rat JE is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 10-30 µg/mL in the presence of 40 ng/mL Recombinant Mouse CCL2/JE/MCP‑1.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. High pressure promotes monocyte adhesion to the vascular wall. Authors: Riou S, Mees B, Esposito B, Merval R, Vilar J, Stengel D, Ninio E, van Haperen R, de Crom R, Tedgui A, Lehoux S Circ. Res., 2007;100(8):1226-33. Species: Mouse Sample Type: Whole Tissue Application: IHC Protection against inflammation- and autoantibody-caused fetal loss by the chemokine decoy receptor D6. Authors: Martinez de la Torre Y, Buracchi C, Borroni EM, Dupor J, Bonecchi R, Nebuloni M, Pasqualini F, Doni A, Lauri E, Agostinis C, Bulla R, Cook DN, Haribabu B, Meroni P, Rukavina D, Vago L, Tedesco F, Vecchi A, Lira SA, Locati M, Mantovani A Proc. Natl. Acad. Sci. U.S.A., 2007;104(7):2319-24. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified
免疫原	E. coli-derived recombinant mouse CCL2/JE/MCP-1 Gln24-Arg96 Accession # Q5SVU3
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Rollins, B.J.et al. (1988) Proc. Natl. Acad. Sci. USA 85:3738. Gu, L.et al. (1999) Chem. Immunol. 72:7. Luini, W.et al. (1994) Cytokine 6:28.

参考图片

Chemotaxis Induced by CCL2/JE/MCP‑1 and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE/MCP‑1 Polyclonal Antibody (Catalog # AB-479-NA). The ND₅₀ is typically 10-30 µg/mL.