Mouse IL-10 Polyclonal Ab (1 MG)【科瑞奥博】

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Mouse IL-10 Polyclonal Ab (1 MG)

货号： AB-417-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-417-NA
别名	CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF
全称	Interleukin 10
反应种属	Mouse
应用	Western Blot(1 µg/mL)
目标/特异性	Detects mouse IL-10 in Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human IL‑10 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line [Thompson-Snipes, L. et al. (1991) J. Exp. Med. 173:507]. The Neutralization Dose (ND₅₀) is typically 2-4 µg/mL in the presence of 2.5 ng/mL Recombinant Mouse IL‑10.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Platelets provoke distinct dynamics of immune responses by differentially regulating CD4+ T-cell proliferation. Authors: Zhu L, Huang Z, Stalesen R, Hansson G, Li N J Thromb Haemost, 2014;12(7):1156-65. Species: Human Sample Type: Whole Cells Application: Bioassay Low-Dose Lipopolysaccharide Pretreatment Suppresses Choroidal Neovascularization via IL-10 Induction. Authors: Matsumura N, Kamei M, Tsujikawa M PLoS ONE, 2012;7(7):e39890. Species: Mouse Sample Type: In Vivo Application: In Vivo Histone Deacetylase Inhibitor LAQ824 Augments Inflammatory Responses in Macrophages through Transcriptional Regulation of IL-10. Authors: Wang H, Cheng F J. Immunol., 2011;186(0):3986. Species: Mouse Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified
免疫原	E. coli-derived recombinant mouse IL‑10 Ser19-Ser178 Accession # NP_034678
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL-22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature mouse IL-10 shares 85% amino acid sequence identity with rat and 70%‑77% with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL-10 R alpha /IL-10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10 R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL-10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL‑29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172. Hsu, D.-H. et al. (1990) Science 250:830. Moore, K. et al. (1990) Science 248:1230. Windsor, W.T. et al. (1993) Biochemistry 32:8807. Syto, R. et al. (1998) Biochemistry 37:16943. Kotenko, S.V. et al. (1997) EMBO J. 16:5894. Kotenko, S.V.et al. (2000) J. Biol. Chem. 276:2725. Hor, S. et al. (2004) J. Biol. Chem. 279:33343. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.

参考图片

Cell Proliferation Induced by IL‑10 and Neutralization by Mouse IL‑10 Antibody. Recombinant Mouse IL‑10 (Catalog # 417-ML) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑10 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse IL‑10 Polyclonal Antibody (Catalog # AB-417-NA). The ND₅₀ is typically 2-4 µg/mL.