Mouse TNF-alpha Polyclonal Ab (1 MG)【科瑞奥博】

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Mouse TNF-alpha Polyclonal Ab (1 MG)

货号： AB-410-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-410-NA
别名	APC1 protein; Cachectin; Cachetin; DIF; TNF; TNF, monocyte-derived; tnfa; tnf-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF1A; TNFSF2; TNFSF2TNF superfamily, member 2; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor; tumor necrosis factor-alpha
全称	Tumor Necrosis Factor alpha
反应种属	Mouse
应用	Western Blot(1 µg/mL)
目标/特异性	Detects mouse TNF-alpha in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with human TNF‑ alpha is observed, and less than 5% cross-reactivity with recombinant bovine TNF‑ alpha, recombinant canine TNF‑ alpha, recombinant equine TNF‑ alpha, recombinant feline TNF‑ alpha, and recombinant porcine TNF‑ alpha is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize TNF‑ alpha -induced cytotoxicity in the L‑929 mouse fibroblast cell line [Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221]. The Neutralization Dose (ND₅₀) is typically 0.2-0.6 µg/mL in the presence of 0.25 ng/mL Recombinant Mouse TNF‑ alpha and 1 µg/mL actinomycin D.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	7124 (Human); 21926 (Mouse); 24835 (Rat); 397086 (Porcine); 280943 (Bovine); 403922 (Canine); 100033834 (Equine); 493755 (Feline); 100009088 (Rabbit)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Distinct mechanisms of induction of hepatic growth hormone resistance by endogenous IL-6, TNF-alpha, and IL-1beta. Authors: Zhao Y, Xiao X, Frank S, Lin H, Xia Y Am J Physiol Endocrinol Metab, 2014;307(2):E186-98. Species: Mouse Sample Type: In Vivo Application: In vivo Mammalian clock gene Cryptochrome regulates arthritis via proinflammatory cytokine TNF-alpha. Authors: Hashiramoto A, Yamane T, Tsumiyama K, Yoshida K, Komai K, Yamada H, Yamazaki F, Doi M, Okamura H, Shiozawa S J. Immunol., 2010;184(3):1560-5. Species: Mouse Sample Type: In Vivo Application: In vivo Long-lasting neuropathic pain induced by brachial plexus injury in mice: Role triggered by the pro-inflammatory cytokine, tumour necrosis factor alpha. Authors: Quintao NL, Balz D, Santos AR, Campos MM, Calixto JB Neuropharmacology, 2006;50(5):614-20. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified
免疫原	E. coli-derived recombinant mouse TNF-alpha Leu80-Leu235 Accession # P06804
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Mouse
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	Tumor necrosis factor alpha (TNF-alpha, also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. TNF-alpha is produced by a wide variety of immune and epithelial cell types (1, 2). Mouse TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (3). Within the ECD, mouse TNF-alpha shares 94% aa sequence identity with rat and 70% - 77% with bovine, canine, cotton rat, equine, feline, human, porcine, rat, and rhesus TNF-alpha. The 26 kDa type 2 transmembrane protein is assembled intracellularly to form a noncovalently linked homotrimer (4). Ligation of this complex induces reverse signaling that promotes lymphocyte costimulation but diminishes monocyte responsiveness (5). Cleavage of membrane bound TNF-alpha by TACE/ADAM17 releases a 55 kDa soluble trimeric form of TNF-alpha (6, 7). TNF-alpha trimers bind the ubiquitous TNF RI and the hematopoietic cell-restricted TNF RII, both of which are also expressed as homotrimers (1, 8). TNF-alpha regulates lymphoid tissue development through control of apoptosis (2). It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages (2). TNF-alpha is a key cytokine in the development of several inflammatory disorders (9). It contributes to the development of type 2 diabetes through its effects on insulin resistance and fatty acid metabolism (10, 11).
运输条件	Blue Ice
存放说明	-20℃
参考文献	Idriss, H.T. and J.H. Naismith (2000) Microsc. Res. Tech. 50:184. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1. Fransen, L. et al. (1985) Nucl. Acids Res. 13:4417. Tang, P. et al. (1996) Biochemistry 35:8216. Eissner G. et al. (2004) Cytokine Growth Factor Rev. 15:353. Black, R.A. et al. (1997) Nature 385:729. Moss, M.L. et al. (1997) Nature 385:733. Loetscher, H. et al. (1991) J. Biol. Chem. 266:18324. Clark, I.A. (2007) Cytokine Growth Factor Rev. 18:335. Romanatto, T. et al. (2007) Peptides 28:1050. Hector, J. et al. (2007) Horm. Metab. Res. 39:250.

参考图片

Cytotoxicity Induced by TNF‑ alpha and Neutralization by Mouse TNF‑ alpha Antibody. Recombinant Mouse TNF‑ alpha (Catalog # 410-MT) induces cytotoxicity in the the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Cytotoxicity elicited by Recombinant Mouse TNF‑ alpha (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse TNF‑ alpha Polyclonal Antibody (Catalog # AB-410-NA). The ND₅₀ is typically 0.2-0.6 µg/mL in the presence of the metabolic inhibitor actinomycin D (1 µg/mL).