Human CCL7/MCP-3 Polyclonal Ab (1 MG)【科瑞奥博】

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Human CCL7/MCP-3 Polyclonal Ab (1 MG)

货号： AB-282-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-282-NA
别名	C-C motif chemokine 7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human CCL7/MCP‑3/MARC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 6% cross-reactivity with recombinant human (rh) MCP-2 and rhMCP-1 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in human monocytes. The Neutralization Dose (ND₅₀) is typically 30-60 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL7/MCP‑3/MARC.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6354 (Human); 20306 (Mouse)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Let-7d suppresses growth, metastasis, and tumor macrophage infiltration in renal cell carcinoma by targeting COL3A1 and CCL7. Authors: Su, Boxing, Zhao, Wei, Shi, Bentao, Zhang, Zhongyua, Yu, Xi, Xie, Feng, Guo, Zhongqia, Zhang, Xiaoyu, Liu, Jin, Shen, Qi, Wang, Jinghua, Li, Xuesong, Zhang, Zhiqian, Zhou, Liqun Mol Cancer, 2014;13(0):206. Species: Human Sample Type: Whole Cells Application: Neut
纯化方式	Protein A or G purified
免疫原	E. coli-derived recombinant human CCL7/MCP‑3/MARC Gln24-109 Accession # P80098
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2. CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites. Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
运输条件	Blue Ice
存放说明	-20℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Chemokines and Receptors in Angiogenesis Inflammatory Mediators Th22 Cells

参考图片

Chemotaxis Induced by CCL7/MCP‑3 and Neutralization by Human CCL7/MCP‑3 Antibody. Recombinant Human CCL7/
MCP‑3 (Catalog # 282‑P3) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL7/MCP‑3 (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL7/
MCP‑3 Polyclonal Antibody (Catalog # AB-282-NA). The ND₅₀ is typically 30-60 µg/mL.