Human CCL8/MCP-2 Polyclonal Ab (1 MG)【科瑞奥博】

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Human CCL8/MCP-2 Polyclonal Ab (1 MG)

货号： AB-281-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-281-NA
别名	C-C motif chemokine 8; chemokine (C-C motif) ligand 8; HC14; MCP2; MCP-2; member 8 (monocyte chemotactic protein 2); monocyte chemoattractant protein 2; SCYA10; small-inducible cytokine A8
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human CCL8/MCP‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross-reactivity with recombinant human (rh) MCP-1 and rhMCP-3 is observed. Neutralizes the biological activity of recombinant human MCP-2, but will not neutralize the biological activity of MCP-1 or MCP-3.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL8/MCP‑2-induced chemotaxis in human monocytes. The Neutralization Dose (ND₅₀) is typically 60-120 µg/mL in the presence of 0.4 µg/mL Recombinant Human CCL8/MCP‑2.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	6355 (Human); 20307 (Mouse)
纯化方式	Protein A or G purified
免疫原	E. coli-derived recombinant human CCL8/MCP-2 Gln24-Pro99 Accession # P80075
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	MCP-2 and MCP-3 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and MCP-3 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. MCP-3 also shares 58% amino acid identity with MCP-2. Similar to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.
运输条件	Blue Ice
存放说明	-20℃
参考文献	CC Chemokines and Receptors Chemokines and Receptors Chemokines and Receptors in Allergy and Asthma Infectious Diseases Inflammatory Mediators Macrophage Activation Markers

参考图片

Chemotaxis Induced by CCL8/MCP‑2 and Neutralization by Human CCL8/
MCP‑2 Antibody. Recombinant Human CCL8/MCP‑2 (Catalog # 281-CP) chemoattracts human monocytes in a dose-dependent manner (orange line). The amount of cells that migrated through the filter was measured by LeukoStat™ staining (Fisher Scientific). Chemotaxis elicited by Recombinant Human CCL8/MCP‑2 (0.4 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL8/MCP‑2 Polyclonal Antibody (Catalog # AB-281-NA). The ND₅₀ is typically 60‑120 µg/mL.