| 货号 | AB-279-NA |
| 别名 | MCAF; MCP-1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CCL2/JE/MCP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant canine MCP-1 is observed, and less than 1% cross-reactivity with recombinant human (rh) MCP-2, rhMCP-3, rhMCP-4, and recombinant mouse MCP-5 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize CCL2/JE/MCP‑1-induced chemotaxis in BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND50) is typically 50-100 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL2/JE/MCP‑1. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6347 (Human); 20296 (Mouse); 24770 (Rat); 403981 (Canine) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Bone marrow stromal cells derived MCP-1 reverses the inhibitory effects of multiple myeloma cells on osteoclastogenesis by upregulating the RANK expression. | |
| 纯化方式 | Protein A or G purified |
| 免疫原 | E. coli-derived recombinant human CCL2/JE/MCP-1 Gln24-Thr99 Accession # P13500 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | CCL2, also called monocyte chemotactic protein-1 (MCP-1) or JE, is a member of the C-C or beta chemokine family that is best known as a chemotactic agent for mononuclear cells (1, 2). Human CCL2 cDNA encodes a 99 amino acid (aa) precursor protein with a 23 aa signal peptide and a 76 aa mature protein (2). Removal of the first 5 aa of the mature protein, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, occurs naturally by metalloproteinase cleavage and downregulates activity but not receptor binding (3). CCL2 may form multiple bands from 8.7‑13.5 kDa on SDS-PAGE due to non-covalent dimerization and variable carbohydrate content (3). Mature human CCL2 shares 78 - 79% aa identity with canine, porcine and equine CCL2, while mouse and rat express a form of CCL2 that is extended by 49 aa and shares only ~56% aa identity within the common region. Human CCL2 can, however, induce a response in murine cells (4). Fibroblasts, glioma cells, smooth muscle cells, endothelial cells, lymphocytes and mononuclear phagocytes can produce CCL2 either constitutively or upon mitogenic stimulation, but monocytes and macrophages appear to be the major source (1, 2). In addition to its chemotactic activity, CCL2 induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils that express its receptor, CCR2 (2). Additionally, it promotes Th2 polarization in CD4+ T cells (5). CCL2-mediated recruitment of monocytes to sites of inflammation is proposed to play a role in the pathology of atherosclerosis, multiple sclerosis and allergic asthma (6, 7). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
Chemotaxis Induced by CCL2/MCP‑1 and Neutralization by Human CCL2/ MCP‑1 Antibody. Recombinant Human CCL2/
|
