| 货号 | AB-257-NA |
| 别名 | ciliary neurotrophic factor; HCNTF | 全称 | Ciliary Neurotrophic Factor |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human CNTF in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize the effect of CNTF on dorsal root ganglion neurons from E10 chick embryos. Davies, A. M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R. A. (eds): John Willey and Sons, Ltd. 95. The Neutralization Dose (ND50) is typically 15-30 µg/mL in the presence of 10 ng/mL Recombinant Human CNTF. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1270 (Human); 25707 (Rat) |
| 纯化方式 | Protein A or G purified |
| 免疫原 | E. coli-derived recombinant human CNTF Met1-Met200 Accession # P26441 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | Ciliary neurotrophic factor (CNTF) is structurally related to IL-6, IL-11, LIF, CLC, and OSM. CNTF was initially identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types, including dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Neuron Survival in Response to CNTF and Neutralization by Human CNTF Antibody. Recombinant Human CNTF (Catalog # 257-NT) supports the survival of dorsal root ganglion neurons from E10 chick embryos in a dose-dependent manner (orange line), as measured by MTT staining. Neuron survival elicited by Recombinant Human CNTF (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CNTF Polyclonal Antibody (Catalog # AB-257-NA). The ND50 is typically 15‑30 µg/mL. |
