| 货号 | AB-256-NA |
| 别名 | Beta nerve growth factor precursor; beta-nerve growth factor; Beta-NGF; HSAN5; MGC161426; nerve growth factor (beta polypeptide); nerve growth factor, beta subunit; NGFB; NGFBMGC161428 | 全称 | beta Nerve Growth Factor |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human beta -NGF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human BDNF is observed. |
| 使用方法 | Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize beta ‑NGF-induced proliferation in the TF‑1 human erythroleukemic cell line [Kitamura, T. et al. (1989) J. Cell Physiol. 140:323]. The Neutralization Dose (ND50) is typically 10-30 µg/mL in the presence of 5 ng/mL Recombinant Human beta ‑NGF. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 4803 (Human); 18049 (Mouse); 310738 (Rat) |
| 纯化方式 | Protein A or G purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human beta -NGF Ser122-Ala241 Accession # CAA36832 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | NGF was initially isolated in the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. Both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF or 2.5S NGF, exhibits all the biological activities ascribed to NGF. Recombinant human beta -NGF is a homodimer of two 120 amino acid polypeptides. The human protein shares approximately 90% homology at the amino acid level with both the mouse and rat beta -NGF and exhibits cross-species activity. NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor-dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by beta ‑NGF and Neutralization by Human beta ‑NGF Antibody. Recombinant Human beta ‑NGF (Catalog # 256-GF) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human beta ‑NGF (5 ng/mL) is neutralized (green line) by increasing concentrations of Human beta -NGF Polyclonal Antibody (Catalog # AB-256-NA). The ND50 is typically 10-30 µg/mL. |
