| 货号 | AB-239-NA |
| 别名 | protransforming growth factor alpha; TFGA; TGFA; transforming growth factor, alpha; transforming growth factor-alpha | 全称 | Transforming Growth Factor alpha |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TGF‑ alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) AR, rhBTC, rhEGF, rhHB-EGF, rhHRG-alpha, and rhHRG-beta is observed. Neutralizes the biological activity of rhTGF-alpha, but will not neutralize the biological activity of rhEGF. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize TGF‑ alpha -induced proliferation in the Balb/3T3 mouse embryonic fibroblast cell line. The Neutralization Dose (ND50) is typically 0.4-0.8 µg/mL in the presence of 3 ng/mL Recombinant Human TGF‑ alpha. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 7039 (Human) |
| 纯化方式 | Protein A or G purified |
| 免疫原 | E. coli-derived recombinant human TGF-alpha Val40-Ala89 Accession # P01135 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 1 mg/mL in sterile PBS. |
| 背景 | TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects. TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by TGF‑ alpha and Neutralization by Human TGF‑ alpha Antibody. Recombinant Human TGF‑ alpha (Catalog # 239-A) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TGF‑ alpha (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human TGF‑ alpha Polyclonal Antibody (Catalog # AB-239-NA). The ND50 is typically 0.4‑0.8 µg/mL. |
