Human FGF basic Polyclonal Ab (1 MG)【科瑞奥博】

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Human FGF basic Polyclonal Ab (1 MG)

货号： AB-233-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-233-NA
别名	basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF2; FGF-2; FGF2AS; FGFBprostatropin; fibroblast growth factor 2 (basic); GFG1; HBGF-2; HBGH-2; heparin-binding growth factor 2; NUDT6; Prostatropin
全称	Fibroblast Growth Factor basic
反应种属	Human
应用	Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL)
目标/特异性	Detects human FGF basic in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human FGF acidic is observed. Neutralizes the biological activity of recombinant human FGF basic. It will also neutralize the biological effects of bovine FGF basic, but it has no effect on recombinant human or bovine FGF acidic.
使用方法	Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize FGF basic-induced proliferation in the NR6R‑3T3 mouse fibroblast cell line. Rizzino, A. et al. (1988) Cancer Res. 48:4266. The Neutralization Dose (ND₅₀) is typically 0.5-1.0 µg/mL in the presence of 0.5 ng/mL Recombinant Human FGF basic 146 aa.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
应用文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Tricyclic antidepressant amitriptyline indirectly increases the proliferation of adult dentate gyrus-derived neural precursors: an involvement of astrocytes. Authors: Boku, Shuken, Hisaoka-Nakashima, Kazue, Nakagawa, Shin, Kato, Akiko, Kajitani, Naoto, Inoue, Takeshi, Kusumi, Ichiro, Takebayashi, Minoru PLoS ONE, 2013;8(11):e79371. Species: Rat Sample Type: whole cells Application: Neut Improvement in disability after alemtuzumab treatment of multiple sclerosis is associated with neuroprotective autoimmunity. Authors: Jones JL, Anderson JM, Phuah CL, Fox EJ, Selmaj K, Margolin D, Lake SL, Palmer J, Thompson SJ, Wilkins A, Webber DJ, Compston DA, Coles AJ Brain, 2010;133(0):2232-47. Species: Human Sample Type: Whole Cells Application: Neut Dedicated epithelial recipient cells determine pigmentation patterns. Authors: Weiner L, Han R, Scicchitano BM, Li J, Hasegawa K, Grossi M, Lee D, Brissette JL Cell, 2007;130(5):932-42. Species: Mouse Sample Type: In Vivo Application: In vivo
纯化方式	Protein A or G purified
免疫原	E. coli-derived recombinant human FGF basic
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike theother members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic hasbeen isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues,but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. A variety of forms of FGF basic are producedas a result of Nterminal extensions. These extensions affect localization of FGF basic in cellular compartments but do not affect biological activity. Binding of FGFto heparin or cell surface heparan sulfate proteoglycans is necessary for binding of FGF to high affinity FGF receptors. FGF acidic and basic appear to bind to thesame high affinity receptors and show a similar range of biological activities. FGF basic stimulates the proliferation of all cells of mesodermal origin and many cellsof neuroectodermal, ectodermal, and endodermal origin. FGF basic induces neuron differentiation, survival, and regeneration. FGF basic also modulates embryonicdevelopment and differentiation. These observed in vitro functions of FGF basic suggest FGF basic may play a role in vivo in the modulation of such normalprocesses as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, and neuronal function and neural degeneration. Additionally,FGF basic may participate in the production of a variety of pathological conditions resulting from excessive cell proliferation and excessive angiogenesis.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Coulier, F. et al. (1997) J. Mol. Evol. 44:43. Chen, C.H. et al. (2004) Curr. Vasc. Pharmacol. 2:33. Mohammadi, M. et al. (2005) Curr. Opin. Struct. Biol. 15:506. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.

参考图片

Cell Proliferation Induced by FGF basic and Neutralization by Human FGF basic Antibody. Recombinant Human FGF basic 146 aa (Catalog # 233-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human FGF basic 146 aa (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF basic Polyclonal Antibody (Catalog # AB-233-NA). The ND₅₀ is typically 0.5‑1.0 µg/mL.