Human IL-7 Polyclonal Ab (1 MG)【科瑞奥博】

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Human IL-7 Polyclonal Ab (1 MG)

货号： AB-207-NA 基本售价： 4996.9 元规格： -

产品信息

概述
货号	AB-207-NA
别名	IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF
全称	Interleukin 7
反应种属	Human
应用	Western Blot(1 µg/mL)
目标/特异性	Detects human IL-7 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant mouse (rm) IL-7 is observed.
使用方法	Western Blot: 1 µg/mL Neutralization: Measured by its ability to neutralize IL‑7-induced proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The Neutralization Dose (ND₅₀) is typically 0.25-1.25 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑7.
来源	Polyclonal Goat IgG
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	3574 (Human); 16196 (Mouse); 25647 (Rat)
纯化方式	Protein A or G purified
免疫原	E. coli-derived recombinant human IL-7 Asp26-His177 Accession # P13232
内毒素水平	<0.10 EU per 1 μg of the antibody by the LAL method.
生物活性	Human
标记	Unconjugated
溶解方法	Reconstitute at 1 mg/mL in sterile PBS.
背景	IL-7, previously known as pre-B-cell growth factor and lymphopoietin-1, was originally purified on the basis of its ability to promote the proliferation of precursor B‑cells. It has now been shown that IL-7 can also stimulate the proliferation of thymocytes, T cell progenitors and mature CD4⁺ and CD8⁺ T cells. IL-7 can induce the formation of lymphokine-activated killer (LAK) cells as well as the development of cytotoxic T lymphocytes (CTL). IL-7 was also shown to induce the V(D)J rearrangement of the T cell receptor beta gene in mouse fetal thymocytes. Among myeloid lineage cells, IL-7 can up-regulate the production of pro-inflammatory cytokines and stimulate the tumoricidal activity of monocytes/macrophages. IL-7 is expressed by adherent stromal cells from various tissues. Human IL-7 cDNA encodes a precursor protein of 177 amino residues containing a 25 amino acid residue signal peptide. Mouse IL-7 has approximately 65% amino acid sequence identity with human IL-7 and both proteins exhibit cross-species activity. IL-7 bioactivites are mediated by the binding of IL-7 to functional high-affinity receptor complexes. The ligand binding subunit (IL-7 R) of the IL-7 receptor complex has been cloned from human and mouse sources. In addition to the membrane-anchored form of the IL-7 receptor, a human cDNA clone that encodes a soluble form of the IL-7 R has also been isolated. The gamma chain of the IL-2 receptor complex has been shown to be an essential component for IL-7 signal transduction. Both IL-7 R and IL-2 R gamma are members of the hematopoietin receptor superfamily. Cells known to express IL-7 receptors include pre-B cells, T cells and bone marrow cells.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Acute Lymphoblastic Leukemia BIObrief: Common gamma-Chain Family Cytokines Play a Pivotal Role in Regulating Immune System Functions Common gamma Chain Receptor Family Common gamma-Chain Family Cytokines Play a Pivotal Role in Regulating Immune System Functions Interactive Pathway: IL-7 Signaling Pathways Interleukin-7 Inhibits Lymphocyte Apoptosis & Immunosuppression to Improve Survival in a Mouse Model of Sepsis Isolation, Culture, and Identification of Natural Killer Cells Multiple Myeloma Natural Killer T (NKT) Cells NCR-ILC3 Platelets Th2 Cells

参考图片

Cell Proliferation Induced by IL‑7 and Neutralization by Human IL‑7 Antibody. Recombinant Human IL‑7 (Catalog # 207-IL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑7 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑7 Polyclonal Antibody (Catalog # AB-207-NA). The ND₅₀ is typically 0.25‑1.25 µg/mL.