| 货号 | AF8524 |
| 别名 | cadherin 17, LI cadherin (liver-intestine); cadherin; cadherin-16; cadherin-17; CDH16; CDH17; FLJ26931; HPT-1 cadherin; HPT1; HPT-1; human intestinal peptide-associated transporter HPT-1; human peptide transporter 1; Intestinal peptide-associated transporter HPT-1; LI cadherin; LI-cadherin; Liver-intestine cadherin; MGC138218; MGC142024 |
| 反应种属 | Mouse |
| 应用 | Western Blot(1:1000 dilution) Simple Western(1:100 dilution) Immunohistochemistry(1:250 dilution) |
| 目标/特异性 | Detects mouse and human Cadherin-17 in direct ELISAs and Western blots. In direct ELISAs, less then 10% cross-reactivity with recombinant human Cadherin-17 is observed. |
| 使用方法 | Western Blot: 1:1000 dilution Simple Western: 1:100 dilution Immunohistochemistry: 1:250 dilution |
| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1015 (Human); 12557 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Cadherin-17 Glu22-Met786 Accession # Q9R100 |
| 生物活性 | Human, Mouse |
| 标记 | Unconjugated |
| 背景 | Cadherin-17, also known as liver-intestine (LI) Cadherin, belongs to the cadherin family of calcium-dependent cell adhesion molecules. It associates homotypically and is selectively expressed on gastric and intestinal mucosa. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human and Mouse Cadherin‑17 by Western Blot. Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cadherin‑17 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Cadherin‑17 in Mouse Intestine. Cadherin‑17 was detected in perfusion fixed frozen sections of mouse intestine using Rabbit Anti-Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524) at 1:250 dilution overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to the plasma membrane of glandular cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections. | |
Detection of Human and mouse Cadherin‑17 by Simple WesternTM. Simple Western lane view shows lysates of human colon tissue and mouse colon tissue, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin‑17 at approximately 152 and 146 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human/Mouse Cadherin‑17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8524). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
