| 货号 | AF3838 |
| 别名 | antitrypsin), member 6; CBG; CBGcorticosteroid-binding globulin; corticosteroid binding globulin; member 6; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); transcortin |
| 反应种属 | Rat |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects rat Serpin A6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse (rm) Serpin A1, rmSerpin A5, and recombinant human Serpin A4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 866 (Human); 12401 (Mouse); 299270 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant rat Serpin A6 Ser23-Ala396 Accession # NP_001009663 |
| 生物活性 | Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Serpin A6 (also corticosteroid-binding globulin/CBG and Transcortin) is a 45‑60 kDa, secreted glycoprotein member of the clade A serpin family of protease inhibitors. Although it is a serpin family member, it shows no protease inhibition. Serpin A6 is produced principally by hepatocytes, but is also expressed by hypothalamic neurons and anterior pituitary endocrine cells. Serpin A6 transports progesterone and > 90% of circulating cortisol, and likely has three functions: first it can be cleaved, releasing cortisol to the tissues; second, it regulates levels of free hormone available over time; and third, it likely binds to specific cell-surface receptors when complexed to cortisol. Mature rat Serpin A6 is 374 amino acids (aa) in length (aa 23‑396). It contains an RCL segment that is involved in steroid binding (aa 334‑351). Over aa 23‑396, rat Serpin A6 shares 78% and 60% aa sequence identity with mouse and human Serpin A6, respectively. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Rat Serpin A6 by Western Blot. Western blot shows lysates of NRK‑49F rat fibroblasts and rat liver tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Rat Serpin A6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3838) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Serpin A6 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
