Rat Adiponectin/Acrp30 Affinity Purified Polyclonal Ab (100 UG)【科瑞奥博】

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Rat Adiponectin/Acrp30 Affinity Purified Polyclonal Ab (100 UG)

货号： AF3100 基本售价： 4580.5 元规格： -

产品信息

概述
货号	AF3100
别名	ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; adiponectin; adiponectin, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; ApM1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28; GBP28apM1; Gelatin-binding protein
反应种属	Rat
应用	Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunocytochemistry(5-15 µg/mL)
目标/特异性	Detects rat Adiponectin/Acrp30 in direct ELISAs and Western blots. In Western blots, approximately 45% cross-reactivity with recombinant mouse Adiponectin is observed and 35% cross-reactivity with recombinant human Adiponectin is observed.
使用方法	Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunocytochemistry: 5-15 µg/mL
来源	Reconstitute at 0.2 mg/mL in sterile PBS.
产品组分

性能
供应商	R&D Systems
Entrez Gene IDs	9370 (Human); 11450 (Mouse); 246253 (Rat)
纯化方式	Antigen Affinity-purified
免疫原	Mouse myeloma cell line NS0-derived recombinant rat Adiponectin/Acrp30 Ile19-Asn244 Accession # NP_653345
生物活性	Rat
标记	Unconjugated
溶解方法	Reconstitute at 0.2 mg/mL in sterile PBS.
背景	Adiponectin, also known as Adipocyte complement-related protein of 30 kDa (Acrp30), is a secreted adipose tissue-specific protein. It is an anti-atherogenic, anti-diabetic and anti-inflammatory hormone that plays important roles in the regulation of lipid and glucose metabolism. Rat Adiponectin/Acrp30 shares 91% and 83% amino acid sequence homology with mouse and human Adiponectin/Acrp30, respectively.
运输条件	Blue Ice
存放说明	-20℃
参考文献	Adipocytokines Adipogenesis Markers Adiponectin & Apoptotic Cell Clearance Adiponectin & Type 2 Diabetes Adiponectin and Receptors Adiponectin Signaling: The Calcium Connection Cytokines and Receptors in Angiogenesis ELISA For The Measurement of High Molecular Weight Adiponectin Glucose Homeostasis ICC / IHC Images: Adiponectin/Acrp30 Interactive Pathway: Adipocytokines & Insulin Signaling Metabolism Mouse Obesity Antibody Array Obesity Quantikine ELISA Kit for Measuring High Molecular Weight Adiponectin

参考图片

Adiponetin/Acrp30 in Rat Adipocytes. Adiponectin/Adipocyte complement-related protein of 30 kDa (ACRP-30) was detected in rat adipocytes using Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Adiponectin/Acrp30 in Rat Liver. Adiponectin/Acrp30 was detected in perfusion fixed frozen sections of rat liver using Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Adiponectin/Acrp30 in Mouse Adipocytes. Adiponectin/Acrp30 was detected in immersion fixed mouse mesenchymal stem cells differentiated into adipocytes using Rat Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3100) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.