| 货号 | AF1795 |
| 别名 | BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I | 全称 | Interleukin 5 |
| 反应种属 | Feline |
| 应用 | Western Blot(0.1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects feline IL-5 in direct ELISAs and Western blots. In direct ELISAs, approximately 80% cross-reactivity with recombinant bovine IL-5 and recombinant equine IL-5 is observed, approximately 50% cross-reactivity with recombinant porcine IL-5 and recombinant canine IL-5 is observed, approximately 10% cross-reactivity with recombininat human IL-5 is observed, and less than 1% cross-reactivity with recombinant rat IL-5, recombinant mouse IL-5, and recombinant rhesus monkey IL-5 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunocytochemistry: 5-15 µg/mL Neutralization: Measured by its ability to neutralize IL‑5-induced proliferation in the TF‑1 human erythroleukemic cell line. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The Neutralization Dose (ND50) is typically 0.5-2.0 µg/mL in the presence of 30 ng/mL Recombinant Feline IL‑5. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3567 (Human); 16191 (Mouse); 24497 (Rat); 397409 (Porcine); 280825 (Bovine); 403790 (Canine); 493803 (Feline) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant feline IL-5 Ile20-Ser134 Accession # O77515 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Feline |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Interleukin 5 (IL-5) is a T cell-derived factor that promotes the proliferation, differentiation and activation of eosinophils. In mice, IL-5 is also a growth and differentiation factor for B cells (1‑3). Various names previously used to describe IL-5 include: T cell replacing factor (TRF), B cell growth factor II (BCGFII), B cell differentiation factor μ (BCDF μ), eosinophil differentiation factor (EDF) and eosinophil colony-stimulating factor (Eo-CSF). Biologically active IL-5 is a disulfide-linked homodimer. As in human IL-5, the cDNA for cat IL-5 encodes a precursor protein with signal peptide that is cleaved to generate the secreted mature protein containing 115 amino acid (aa) residues. Feline IL-5 shares 70% and 59% aa sequence identity with human and mouse IL-5, respectively. IL-5 exerts its activity on target cells by binding to specific cell surface receptor complexes. The functional high-affinity receptor complex for IL-5 is composed of a ligand-binding alpha subunit that is specific for IL-5, and a non ligand-binding common beta subunit that is required for signal transduction. The common beta subunit is shared with the high-affinity receptor complexes for IL-3 and GM-CSF. In human, IL-5 R alpha subunit is primarily expressed on eosinophils and basophils. During eosinophil development, IL-5 up‑regulates the expression of IL-5 R alpha. In contrast, in mature eosinophils, the expression of IL-5 R alpha mRNA is down-regulated by IL-5, as well as by IL-3 and GM-CSF. Furthermore, IL-5 also down-modulates cell surface IL-5 R alpha via a proteinase-mediated process that releases the soluble IL-5 R alpha extracellular domain (4‑6). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Cell Proliferation Induced by IL‑5 and Neutralization by Feline IL‑5 Antibody. Recombinant Feline IL‑5 (Catalog # 1795-FL) stimulates proliferation in the TF‑1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL‑5 (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Feline IL‑5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1795). The ND50 is typically 0.5-2.0 µg/mL. |
