| 货号 | AF2809 |
| 别名 | Aminopeptidase A; APA; AP-A; CD249 antigen; CD249; Differentiation antigen gp160; EAP; EC 3.4.11; EC 3.4.11.7; ENPEP; glutamyl aminopeptidase (aminopeptidase A); glutamyl aminopeptidase; gp160 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects mouse Aminopeptidase A/ENPEP in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human ENPEP is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 2028 (Human); 13809 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse Aminopeptidase A/ENPEP Arg41-Pro945 Accession # P16406 |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The mouse ENPEP gene encodes Aminopeptidase A (APA), which is also known as glutamyl aminopeptidase (EAP), aspartate aminopeptidase, angiotensinase A, Ca2+-activated glutamate aminopeptidase, membrane aminopeptidase II and the BP-1/6C3 antigen (2). The various names reflect its substrate specificity (prefer Glu or Asp and angiotensin II as one of the physiological substrates), Ca2+-dependence, cellular location (type II membrane protein) and immunological reactivity to antibody BP-1/6C3. The deduced amino acid sequence of mouse ENPEP consists of a short cytoplasmic tail (residues 1 to 18), a transmembrane region (residue 19 to 39), and a long ectodomain (residues 40 to 945) (1). In addition to the N-terminal zinc metalloprotease domain, the ectodomain also contains the C‑terminal region, which functions as an intramolecular chaperone required for the correct folding, cell surface expression and activity (3). The purified rmENPEP consists of the ectodomain and is active in the assay described above. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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