| 货号 | AF2128 |
| 别名 | Antigen NY-CO-27; GAL4; gal-4; galectin 4; galectin-4; L-36 lactose-binding protein; L36LBP; Lactose-binding lectin 4; lectin, galactoside-binding, soluble, 4; LGALS4 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects mouse Galectin-4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 35% cross-reactivity with recombinant human (rh) Galectin-4, approximately 5% cross-reactivity with recombinant mouse (rm) Galectin-3, rmGalectin-7, and rhGalectin-8, and less than 1% cross-reactivity with rmGalectin-1 and rhGalectin-2 is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3960 (Human); 16855 (Mouse) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Brain glycolipids suppress T helper cells and inhibit autoimmune demyelination. | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant mouse Galectin-4 Ala2-Ile326 Accession # Q8K419 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Galectins are a family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins share structural similarities in their carbohydrate recognition domains (CRD), forming three groups often termed prototype (one CRD), tandem-repeat (two CRDs) and chimeric (one CRD, unique N-terminus) (1, 2). All lack classical signal peptides, but are present and active both within and outside of the cell. Galectins are involved in cell adhesion, migration, survival, and apoptosis, and are often up- or down-regulated in cancer (1-3). Galectin-4 is a 36 kDa tandem-repeat galectin found throughout the gastrointestinal tract, but also present in well-differentiated breast and liver carcinomas (3, 4). Each CRD binds a different set of carbohydrate groups, including those found on erythrocyte blood group antigens (3, 5). CRD1 also binds cholesterol 3-sulfate and other sulfatides, which are concentrated within lipid raft membrane microdomains (6, 7). Endocytosed Galectin-4 is thought to play a role in forming the rafts, delivering them to the intestinal apical membrane, and stabilizing highly detergent-resistant "superrafts" (7-9). Human Galectin-4 shares 76%, 77%, 78%, and 80% amino acid (aa) identity with mouse, rat, bovine, and porcine Galectin-4, respectively, with the highest identity occurring within the CRDs. A potential splice variant begins at aa 132 and lacks most of the first CRD (10). Galectin-4 expression is concentrated within microvilli in the gastrointestinal epithelium, where it can interact with CD3 and bind activated T cells in the lamina propria during intestinal inflammation (11, 12). Either pro- or anti-inflammatory activity has been shown, depending on the mouse model used. Galectin-4 can also bind lung, spleen, and kidney macrophages, although its expression is normally low in these tissues (5). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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