| 货号 | AF1026 |
| 别名 | AVP, type 2; CD119 antigen; CD119; FLJ45734; IFN-gamma receptor 1; IFN-gamma-R1; IFNGR; IFNGR1; immune interferon receptor 1; interferon gamma receptor 1; interferon-gamma receptor alpha chain; type 2 | 全称 | Interferon gamma Receptor 1 |
| 反应种属 | Mouse |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects mouse IFN-gamma R1/CD119 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) IFN-gamma RI, recombinant mouse (rm) IFN‑ alpha / beta R2, rmIFN‑ gamma R2, rhIL‑10 R beta, and rhIL‑20 R alpha is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Neutralization: Measured by its ability to neutralize IFN‑ gamma R1/CD119-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M. J. et al. (eds): IRL Press. 129. The Neutralization Dose (ND50) is typically 0.2-1 µg/mL in the presence of 1 ng/mL Recombinant Mouse IFN‑ gamma. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 3459 (Human); 15979 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant mouse IFN‑ gamma R1/CD119 Ala26-Asp253 Accession # Q91Y85 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Mouse |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 (IFN-gamma R alpha ) and IFN-gamma R2 (IFN-gamma R beta ). Both proteins are members of the type II cytokine receptor family and share approximately 52% overall sequence identity. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for IFN-gamma signaling but does not bind IFN-gamma by itself. Human IFN-gamma R1 cDNA encodes a 499 amino acid (aa) residue protein with a 17 aa signal peptide, a 228 aa extracellular domain, a 23 aa transmembrane domain, and a 221 aa intracellular domain. Human and mouse IFN-gamma R1 share 52% amino acid sequence similarity and bind IFN-gamma in a species-specific manner. IFN-gamma R1 is constitutively expressed in most cell types. Soluble IFN-gamma R1 that binds IFN-gamma has been detected in biological fluids. The recombinant soluble IFN-gamma R1 produced at R&D Systems has been shown to bind IFN-gamma with high affinity and is a potent IFN-gamma antagonist. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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IFN‑ gamma Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma R1/CD119 Antibody. Recombinant Mouse IFN‑ gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by Resazurin. Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti‑Mouse IFN‑ gamma R1/CD119 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1026). The ND50 is typically 0.2-1 µg/mL. |
