| 货号 | AF922 |
| 别名 | EDAA2; EDA-A2/Ectodysplasin A2 |
| 反应种属 | Human |
| 应用 | Western Blot(0.1 µg/mL) |
| 目标/特异性 | Detects human EDA‑A2/Ectodysplasin A2 in direct ELISAs and Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhLIGHT, rhOX40 Ligand, and rhTRAIL is observed and less than 2% cross‑reactivity with recombinant mouse EDA, rhTNF-alpha, rhVEGI, rhGITR Ligand, and rhFasLigand is observed. |
| 使用方法 | Western Blot: 0.1 µg/mL Blockade of Receptor-ligand Interaction: In a functional ELISA, 0.3-1.2 µg/mL of this antibody will block 50% of the binding of 5 ng/mL of Recombinant Human EDA-A2 (Catalog # 922-ED) to immobilized Recombinant Human XEDAR Fc Chimera (Catalog # 1093-XD) coated at 1 µg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding. |
| 来源 | Polyclonal Goat IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1896 (Human); 13607 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Mouse myeloma cell line NS0-derived recombinant human EDA‑A2/Ectodysplasin A2 Ala179-Ser389 Accession # NP_001005609 |
| 内毒素水平 | <0.10 EU per 1 μg of the antibody by the LAL method. |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | EDA-A2, a product of the EDA gene (also known as Tabby), is a type II transmembrane protein that is a member of the TNF superfamily. Human EDA-A2 is a 389 amino acid (aa) protein with a predicted N-terminal 39 aa cytoplasmic domain, a 22 aa transmembrane domain and a C-terminal 328 aa extracellular domain. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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