| 货号 | AF6825 |
| 别名 | EC 3.6.1; EC 3.6.4.-; hISWI; hSNF2HSWI/SNF-related matrix-associated actin-dependent regulator of chromatin A5; ISWI; SNF2H; SNF2Hsucrose nonfermenting-like 5; subfamily a, member 5; Sucrose nonfermenting protein 2 homolog; SWI/SNF related, matrix associated, actin dependent regulator of chromatin; SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily A member 5; WCRF135 | 全称 | SWI/SNF-related, Matrix-associated, Actin-dependent Regulator of Chromatin, Subfamily A, Member 5 |
| 反应种属 | Human |
| 应用 | Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human SMARCA5/SNF2H in direct ELISAs. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) SMARCA1 and rhSMARC4 is observed. |
| 使用方法 | Immunohistochemistry: 5-15 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 8467 (Human); 93762 (Mouse); 307766 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SAMRCA5/SNF2H Asp77-Val185 Accession # O60264 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | SMARCA5 (Swi/SNF-related matrix-associated actin-dependent regulator of chromatin, subfamily A, member 5; also SNF2H) is a 130-135 kDa member of the SNF2/RAD54 helicase family of molecules. It is a ubiquitously expressed, chromatin remodeling ATPase that increases the accessibility of nucleosomal DNA to DNA-binding proteins. It apparently does so by sliding histone octamers over DNA. SMARCA5 is a component of four chromatin-remodeling complexes, including CHRAC, RSF, ACF and WICH. Human SMARCA5 is 1052 amino acids (aa) in length. It contains an N-terminal helicase domain (aa 192-357), a helicase superfamily C-terminal domain (aa 474-605), and two SANT DNA-binding domains (aa 840-892 and 943-1007). Over aa 77-185, human SMARCA5 shares 99% aa identity with mouse SMARCA5. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
|
SMARCA5/SNF2H in Human Spleen. SMARCA5/SNF2H was detected in immersion fixed paraffin-embedded sections of human spleen using Goat Anti-Human SMARCA5/SNF2H Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6825) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in splenocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
