| 货号 | AF6817 |
| 别名 | CDK2/cyclin A-associated protein p45; cyclin A/CDK2-associated protein p45; Cyclin-A/CDK2-associated protein p45; FBL1; F-box protein Skp2; F-box/LRR-repeat protein 1; FBXL1; FBXL1MGC1366; FLB1; p45skp2; S-phase kinase-associated protein 2 (p45); S-phase kinase-associated protein 2 | 全称 | S Phase Kinase-associated Protein 2 |
| 反应种属 | Human |
| 应用 | Western Blot(2 µg/mL) |
| 目标/特异性 | Detects human Spk2 in direct ELISAs and Western blots. |
| 使用方法 | Western Blot: 2 µg/mL |
| 来源 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6502 (Human); 27401 (Mouse); 294790 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Skp2 Leu130-Pro350 Accession # Q13309 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Sterile PBS to a final concentration of 0.2 mg/mL. |
| 背景 | Skp2 (S-phase kinase-associated protein 2; also F-box/LRR protein 1) is a 45-49 kDa member of the F-box protein family. It is known to be part of the SCF (Skp1/Cul1/F-box protein) complex. The SCF complex functions as an E3 ubiquitin-protein ligase that mediates the transfer of ubiquitin from an E2 enzyme to a target substrate. The specificity of the target is determined by Skp2. Molecules targeted for degradation include cell-cycle regulatory proteins that are involved in the G1-S phase transition. Human Skp2 is 424 amino acids (aa) in length. It contains one F-box (aa 94-140) followed by nine consecutive Leu-rich repeats (aa 142-369). There are two potential splice forms that contain substitutions for the same aa sequence. One contains a 4 aa substitution, while a second contains a 56 aa substitution for aa 354-424. Over aa 130-350, human Skp2 shares 84% aa identity with mouse Skp2.
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| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Skp2 by Western Blot. Western blot shows lysates of 293T human embryonic kidney cell line. PVDF Membrane was probed with 2 µg/mL of Sheep Anti-Human Skp2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6817) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for Skp2 at approximately 45 and 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
