| 货号 | AF5884 |
| 别名 | Colorectal cancer suppressor; CRC18; CRCR1; deleted in colorectal cancer protein; deleted in colorectal carcinoma; IGDCC1colorectal tumor suppressor; Immunoglobulin superfamily DCC subclass member 1; immunoglobulin superfamily, DCC subclass, member 1; netrin receptor DCC; Tumor suppressor protein DCC | 全称 | Deleted in Colorectal Cancer |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human DCC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) ICAM‑1, rhICAM-2, rhICAM-3, rhMadCAM-1, rhPECAM-1, rhVCAM-1, and rhCEACAM-1 is observed. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1630 (Human); 13176 (Mouse) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DCC Ser1323-Phe1447 Accession # P43146 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Deleted in colorectal cancer (DCC) is a 170-190 kDa transmembrane glycoprotein that contains four Ig-like domains and six fibronectin type III repeats in its extracellular domain. DCC is expressed on axons during development where its binding to Netrin-1 regulates axon migration and myelination. It inhibits apoptosis when bound by Netrin-1 and can induce apoptosis in the absence of ligand. DCC also functions as an adhesion molecule and a tumor suppressor on intestinal epithelial cells. Deficiencies in DCC function are associated with the development and metastasis of many tumors. Within aa 1323-1447 of the cytoplasmic domain, human DCC shares 98% aa sequence identity with the mouse and rat DCC. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human DCC by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and HT‑29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human DCC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5884) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for DCC at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
