| 货号 | AF5596 |
| 别名 | FLJ36364; MGC47735; T-cell factor 1; T-cell specific; TCF1; TCF-7; transcription factor 7 (T-cell specific, HMG-box) | 全称 | Transcription Factor 7 |
| 反应种属 | Human |
| 应用 | Western Blot(0.2 µg/mL) Immunohistochemistry(3-15 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human TCF7/TCF1 in Western blots. |
| 使用方法 | Western Blot: 0.2 µg/mL Immunohistochemistry: 3-15 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 6932 (Human); 21414 (Mouse); 363595 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human TCF7/TCF1 Met116-His233 Accession # P36402 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | TCF7 (Transcription factor 7; also T cell factor 1/TCF1) is a 25‑50 kDa member of the lymphoid enhancer binding factor family of proteins with 16 isoforms. It is expressed in thymocytes and mature T cells, and serves multiple purposes. In resting cells, TCF family members are transcriptional repressors, and are 25‑32 kDa in size. Following activation, large TCF7 isoforms predominate (42‑50 kDa), and serve a transcriptional activator function. Human TCF7 is 384 amino acids (aa) in length. This is likely an activating isoform that contains a beta -catenin binding domain (aa 1‑59), a DNA-binding HMG-box (aa 269‑337), and an NLS (aa 344‑348). The use of an alternate start site at Met116 seems to characterize repressor isoforms. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human TCF7/TCF1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for TCF7/TCF1 at approximately 60, 40, and 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
TCF7/TCF1 in HCT‑116 Human Cell Line. TCF7/TCF1 was detected in immersion fixed HCT‑116 human colorectal carcinoma cell line using Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. | |
TCF7/TCF1 in Human Thymus. TCF7/TCF1 was detected in immersion fixed paraffin-embedded sections of human thymus using Goat Anti-Human TCF7/TCF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5596) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
