| 货号 | AF5357 |
| 别名 | carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 4; CHST4; EC 2.8.2; EC 2.8.2.-; EC 2.8.2.17; EC 2.8.2.21; galactose/N-acetylglucosamine/N-acetylgalactosamine 6-O-sulfotransferase 3; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 3; GlcNAc6ST2; GlcNAc6ST-2; gn6st-2; GST3; GST-3; HECGLCNAC6ST; HEC-GlcNAc6ST; HEC-GLCNAC-6-ST; High endothelial cells N-acetylglucosamine 6-O-sulfotransferase; L-selectin ligand sulfotransferase; LSST; LSSTcarbohydrate sulfotransferase 4; N-acetylglucosamine 6-O-sulfotransferase 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Sulfotransferase 4/CHST4 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross‑reactivity with recombinant mouse CHST4 is observed and less than 5% cross-reactivity with recombinant human (rh) CHST1, rhCHST5, and rhCHST6 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunoprecipitation: 25 µg/mL Neutralization: Measured by its ability to neutralize Recombinant Human Carbohydrate Sulfotransferase 4/CHST4 (1.33 µg/mL, Catalog # 5357-ST) transfer from the sulfate donor Adenosine 3-phosphate 5-phosphosulfate (PAPS, 42 μM) to beta -D-GlcNAc-(1→6)-alpha -D-Man→OMe (833 μM). The Neutralization Dose (ND50) is typically 10.7 µg/mL. |
| 来源 | Polyclonal Sheep IgG |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 10164 (Human); 26887 (Mouse); 307838 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | Chinese hamster ovary cell line CHO-derived recombinant human Sulfotransferase 4/CHST4 Leu22-Gln286 Accession # Q8NCG5 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The CHST family is comprised of 14 enzymes in human. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids. This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses. CHST4, also known as high endothelial cells N-acetylglucosamine 6-O-sulfotransferase (HEC-GlcNAc6ST) or L-selectin ligand sulfotransferases (LSST), catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues within mucin-associated glycans that ultimately serve as L-selectin ligands. It has a catalytic preference for core 2-branched mucin-type O-glycans, but also has activity toward core 3 type of O-glycan. Human CHST4 shares 72% amino acid sequence identity with the mouse ortholog. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Carbohydrate Sulfotransferase 4/CHST4 by Western Blot. Western blot shows lysates of SW13 human adrenal cortex adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbohydrate Sulfotransferase 4/CHST4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5357) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbohydrate Sulfotransferase 4/CHST4 at approximately XXX kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. |
