| 货号 | AF5339 |
| 别名 | dedicator of cytokinesis 2; dedicator of cytokinesis protein 2; FLJ46592; KIAA0209dedicator of cyto-kinesis 2 | 全称 | Dedicator of Cytokinesis Protein 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) |
| 目标/特异性 | Detects human DOCK2 in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 1794 (Human); 94176 (Mouse); 360509 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human DOCK2 Gly420-Gly642 Accession # Q92608 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | DOCK2 (Dedicator of cytokinesis protein 2) is a member of the DOCK-A family, DOCK180 superfamily of proteins. Its predicted molecular weight is 210 kDa, although it may run approximately at 190 kDa in SDS-PAGE. It is found in hematopoietic cell types, and regulates the activity of the GTPases Rac-1 and -2. Human DOCK2 is 1830 amino acids (aa) in length. It contains one SH3 domain (aa 8‑69), a DHR-1 region that localizes DOCK2 to the plasma membrane (aa 420‑662), and a DHR-2 domain that binds to nucleotide-free GTPases and shows GEF activity (aa 1115‑1621). There are two isoform variants, one that shows a 38 aa substitution for Tyr1405, and a second that shows a start site at Met450 with accompanying deletions of aa 462‑494 and 630‑766, and a 22 aa substitution for aa 934‑1830. Over aa 420‑642, human DOCK2 shares 96% aa identity to mouse DOCK2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human DOCK2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, and Raji human Burkitts lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human DOCK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DOCK2 at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
