| 货号 | AF4819 |
| 别名 | FLJ31917; MGC163164; Spred-2related sequence; sprouty-related, EVH1 domain containing 2; sprouty-related, EVH1 domain-containing protein 2 | 全称 | Sprouty-related, EVH1 Domain Containing 2 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human SPRED2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) SPRED1 is observed and less than 3% cross-reactivity with rhSPRED4 is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 200734 (Human); 114716 (Mouse); 305539 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human SPRED2 Lys117-Ser297 Accession # Q7Z698 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | SPRED2 (Sprouty-related protein with an EVH1 domain-2) is a 47-53 kDa member of the SPRED family of regulatory molecules. In human, it is found in keratinocytes, intestinal columnar epithelium, and salivary duct epithelium. It inhibits receptor tyrosine kinase (RTK) signaling by blocking Ras activation of Raf. This is achieved by inducing the lysosomal degradation of RTKs following SPRED2 binding to NBR1. Human SPRED2 is 418 amino acids (aa) in length. It contains an N‑terminal WH1/EVH1 domain that is involved in ERK inhibition (aa 5-122), a central KBD domain that binds to the SCFR (aa 201-257), and a C-terminal Sprouty‑related domain that mediates homodimerization, and heterodimerization with SPRED1 (aa 308-416). SPRED is ubiquitinated, and undergoes phosphorylation on Ser, Thr and Tyr residues. There is one splice variant that shows a six aa substitution for aa 1-9. Over aa 117-297, human SPRED2 shares 84% aa identity with mouse SPRED2. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human SPRED2 by Western Blot. Western blot shows lysates of Hep3B human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Human SPRED2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4819) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SPRED2 at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8. | |
SPRED2 in PC‑12 Rat Cell Line. SPRED2 was detected in immersion fixed PC‑12 rat adrenal pheochromocytoma cell line using Human SPRED2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4819) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
