| 货号 | AF4717 |
| 别名 | ataxia telangiectasia and Rad3 related; Ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein 1; FRP1; FRP1FRAP-related protein-1; MEC1; protein kinase ATR; Rad3 related protein; SCKL1; SCKLMEC1, mitosis entry checkpoint 1, homolog; serine/threonine-protein kinase ATR | 全称 | Ataxia Telangiectasia and Rad 3-related |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects human ATR in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 545 (Human); 245000 (Mouse); 685055 (Rat) |
| 应用文献 | |
| R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions. Time-resolved analysis of DNA-protein interactions in living cells by UV laser pulses | |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human ATR Glu2405-Met2644 Accession # Q13535 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | ATR (Ataxiatelangiectasia and Rad 3-related) is a member of the phosphatidylinositolkinase-relatedkinase (PIKK) family of protein kinases. ATR is a large protein kinase (~300 kDa) that functions in the response to genotoxic stress, DNA recombination, and cell cycle control. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human ATR by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for ATR at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
ATR in Human Ovarian Cancer Tissue. ATR was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human ATR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4717) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. |
