| 货号 | AF4030 |
| 别名 | Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like; BCL2/adenovirus E1B 19-kd protein-interacting protein 3a; BCL2/adenovirus E1B 19kDa interacting protein 3-like; BNIP3a; BNIP3H; Nip3L; NIP3-like protein X; Nix; NIXBCL2/adenovirus E1B 19kD-interacting protein 3-like | 全称 | BCL2/Adenovirus E1B 19 Kda Protein-Interacting Protein 3-Like |
| 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1 µg/mL) Immunocytochemistry(5-15 µg/mL) |
| 目标/特异性 | Detects endogenous human, mouse and rat BNIP3L in Western blots. In Western blots, less than 10% cross-reactivity to recombinant human BNIP3L is observed. |
| 使用方法 | Western Blot: 1 µg/mL Immunocytochemistry: 5-15 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 665 (Human); 12177 (Mouse); 140923 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BNIP3L Ser2-Glu184 Accession # O60238 |
| 生物活性 | Human, Mouse, Rat |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) also known as BNIP3 alpha and NIP3-like protein X (Nix), is a proapoptotic member of BNIP3 protein family. BNIP3L is a functional homolog of BNIP3 and both proteins contain a single Bcl-2 homology 3 (BH3) domain. BNIP3L is a 219 amino acid (aa), 24 kDa (predicted) protein that contains a C-terminal transmembrane domain required for mitochondrial localization, homodimerisation, and regulation of its proapoptotic function. BNIP3L may antagonize the activity of BCL2 family antiapoptic proteins by directly interactacting with proteins such as viral E1B-19K and cellular Bcl-2 and Bcl-xL. BNIP3L shares 56% amino acid sequence identity with BNIP3 and 98% with mouse and rat BNIP3L. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human/Mouse/Rat BNIP3L by Western Blot. Western blot shows lysates of Raji human Burkitts lymphoma cell line, A20 mouse B cell lymphoma cell line, and Rat‑2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat BNIP3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4030) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BNIP3L at approximately 35kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2. | |
BNIP3L in HeLa Human Cell Line. BNIP3L was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat BNIP3L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4030) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips. |
