| 货号 | AF2210 |
| 别名 | BRCAI; breast and ovarian cancer susceptibility protein 1; breast and ovarian cancer sususceptibility protein; breast cancer 1, early onset; breast cancer type 1 susceptibility protein; EC 6.3.2; EC 6.3.2.-; IRIS; PNCA4; PSCP; subunit 1 | 全称 | Breast Cancer 1 |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(3 µg/500 µg cell lysate) |
| 目标/特异性 | Detects human BRCA1 N-Terminus in Western blots. |
| 使用方法 | Western Blot: 1 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 3 µg/500 µg cell lysate |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 672 (Human); 12189 (Mouse); 497672 (Rat) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human BRCA1 N-Terminus Met1-Gly200 Accession # P38398 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | The BRCA1 (BReast CAncer gene 1) tumor suppressor protein has many reported functions. In addition to mediating signal transduction in DNA damage and repair responses, BRCA1 regulates transcriptional activity and assists in the preservation of chromosomal stability. BRCA1 is one of the first proteins recruited to sites of DNA double-strand breaks, and serves as part of a scaffold for assembling other DNA damage response or repair factors. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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BRCA1 in Human Breast Cancer Tissue. BRCA1 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using 5 µg/mL Goat Anti-Human BRCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2210) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections. | |
Detection of Human BRCA1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-human BRCA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2210) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for BRCA1 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. |
