| 货号 | AF2184 |
| 别名 | CA2; CAII; CA-IIEC 4.2.1.1; Car2; Carbonate dehydratase II; carbonic anhydrase 2; carbonic anhydrase B; Carbonic anhydrase C; carbonic anhydrase IICAC; carbonic dehydratase |
| 反应种属 | Human |
| 应用 | Western Blot(1 µg/mL) Simple Western(10 µg/mL) Immunohistochemistry(5-15 µg/mL) Immunoprecipitation(25 µg/mL) |
| 目标/特异性 | Detects human Carbonic Anhydrase II/CA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Carbonic Anhydrase I, III, and XIII is observed and less than 1% cross-reactivity with rhCarbonic Anhydrase IV, VIII, IX, X, XII, and XIV is observed. |
| 使用方法 | Western Blot: 1 µg/mL Simple Western: 10 µg/mL Immunohistochemistry: 5-15 µg/mL Immunoprecipitation: 25 µg/mL |
| 来源 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 产品组分 |
| 供应商 | R&D Systems |
| Entrez Gene IDs | 760 (Human) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | E. coli-derived recombinant human Carbonic Anhydrase II/CA2 Ser2-Lys260 Accession # P00918 |
| 生物活性 | Human |
| 标记 | Unconjugated |
| 溶解方法 | Reconstitute at 0.2 mg/mL in sterile PBS. |
| 背景 | Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in the CA meeting (6th International Conference on the CAs, June 20‑25, 2003, Slovakia) ranged from use of CAs as markers for tumor and hypoxia in the clinic, as nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA II deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification (2). |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 |
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Detection of Human Carbonic Anhydrase II/CA2 by Western Blot. Western blot shows lysates of human heart tissue, human kidney tissue, Caki‑2 human clear cell carcinoma epithelial cell line, and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/ CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Carbonic Anhydrase II/ CA2 at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. | |
Detection of Human Carbonic Anhydrase II/CA2 by Simple WesternTM. Simple Western lane view shows lysates of human kidney tissue, human heart tissue, and Caco‑2 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Carbonic Anhydrase II/CA2 at approximately 37 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Carbonic Anhydrase II/CA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2184) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. |
