概述| 货号 | PPS024 |
| 别名 | GABA(A) receptor subunit alpha-2; GABRA2; gamma-aminobutyric acid (GABA) A receptor, alpha 2; gamma-aminobutyric acid receptor subunit alpha-2 |
全称 | gamma-Aminobutyric Acid Type A Receptor, alpha-2 Polypeptide | | 反应种属 | Human/Mouse/Rat |
| 应用 | Western Blot(1:1000 dilution)
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| 目标/特异性 | Human, mouse, rat and bovine ~53 kDa GABAA R alpha 2 subunit |
| 使用方法 | Western Blot: 1:1000 dilution
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| 来源 | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| 产品组分 | |
性能| 供应商 | R&D Systems |
| Entrez Gene IDs | 2555 (Human); 14395 (Mouse); 289606 (Rat); 282236 (Bovine) |
| 纯化方式 | Antigen Affinity-purified |
| 免疫原 | GABAA R alpha 2 subunit specific peptide |
| 生物活性 | Human, Mouse, Rat, Bovine, Canine, Primate |
| 标记 | Unconjugated |
| 背景 | GABAA ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha 2 isoform is a 53 kDa, 423 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 15 - 222). As with many receptors, phosphorylation is likely to be used as a regulatory mechanism. The alpha 2 subunit is known to be phosphorylated on Ser/Thr residues. Rat alpha 2 has multiple isoforms, all involving the 5′ UTR. These may dictate alpha 2 levels within the cell. This receptor subunit is particularly abundant in forebrain areas and is a potential target of anabolic steroids. Behavioral alterations associated with steroid abuse may be a manifestation of alpha 2 beta 3 gamma 2L receptor activation. |
| 运输条件 | Blue Ice |
| 存放说明 | -20℃ |
| 参考文献 | - Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
- Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
- Khrestchatisky, M. et al. (1991) J. Neurochem. 56:1717.
- Yang, P. et al. (2005) Neuropharmacology 49:300.
- Tian, H. et al. (2005) Mol. Brain Res. 137:174.
- Bureau, M.H. and J.J. Laschet (1995) J.Biol. Chem. 270:26482.
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参考图片Detection of GABAA R alpha 2 by Western Blot. Western blot of 10 μg of rat cortex lysates showing immunolabeling of the ~53 kDa alpha 2 subunit of the GABAAR. |
